Insoluble polymeric diazonium salt chromogen

Abstract

Abstract A NOVEL INSOLUBLE POLYMERIC DIAZONIUM SALT CHROMOGEN CONSISTING OF A DIAZOTIZED AROMATUIC POLYAMINE BOUND BY AN AMIDE LINKAGE TO A CATION EXCHANGES RESIN CAN BE PREPARED BY REACTING A CATION EXCHANGE RESIN CONTAINING CARBOXYLIC ACID GROUPS OR SALTS OF SUCH CARBOXYLIC ACID GROUPS WITH A HALOGENATING AGENT TO CONVERT SOME OF THE RESIN CARBOXYLIC ACID GROUPS OR SALTS OF SUCH ACID GROUPS TO ACYL HALIDE GROUPS, REACTING THE RESULTING ACYL HALIDE GROUPS WITH AN AROMATIC POLYAMINE TO FORM A RESIN CONTAINING AMIDE LINKAGES AND FREE AROMATIC AMINO GROUPS AND THEN DIAZOTIZING THE FREE AROMATIC AMINO GROUPS, THE RESULTING INSOLUBLE CHROMOGEN CAN BE USED TO DETECT COUPLING COMPOUNDS BY FORMING COLORED REACTION PRODUCTS WITH SUCH COUPLING COMPOUNDS. THE CHROMOGEN CAN ALSO BE USED TO DETECT ENZYME ACTIVITY WHEREIN THE ENZYME CATALYZES A REACTION WITH A SUITABLE SUBSTRATE TO RELEASE A COUPLING COMPOUND WHICH WILL FORM A COLORED REACTION PRODUCT WITH THE INSOLUBLE CHROMOGEN.

Inventors

• 👤 G Linoli
• 👤 E Mannucci

Bibliographic Details