Method for determining total blood serum iron-binding capacity
Abstract
Abstract A METHOD FOR DETERMINING THE TOTAL IRONN-BINDING CAPACITY OF BLOOD SERUM. THE METHOD COMPRISES FIRST RELEASING THE IRON NORMALLY PRESENT ON THE SERUM AND KNOWN AS THE SERUM IRON (SI), THE FREE IRONN IS THEN REMOVED TO PREVENT IT FROM RECOMBINING WITH THE SERUM. NEXT, THE SERUM IS SATURATED WITH A TRACER AMOUNT OF RADIOACTIVE IRON, AN ION-EXCHANGE RESIN IS MIXED WITH THE SERUMRADIOACTIVE IRON MIXTURE AND THE COMBINED MIXTURE IS INCUBATED. AFTER INCUBATION, THE INITIAL RADIOACTIVITY OF THE MIXTURE AND ION-EXCHANGE RESIN IS MEASURED WITH SUITABLE DETECTING MEANS, AFTER WHICH ALL OF THE LIQUID IS REMOVED. THE ION-EXCHANGE RESIN IS THEN WASHED AND THE RESIDUAL RRADIOACTIVITY DETERINED. BY SUITABLE CALCULATION, THE TOTAL IRON-BINDING CAPACITY OF THE SERUM IS THEN DETERMINED.
Inventors
- 👤 C Burke
Bibliographic Details
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