Heteroploid cell lines
Abstract
Abstract A HETEROPLOID CELL LINE PRODUCED BY CULTURING AND SUBCULTURING A DIPLOID CELL LINE IN A MEDIUM PROVIDING THE NECESSARY NUTRITIONAL COMPOSITION SUCH THAT THE TOTAL PASSAGING OF THE CELL LINE EXCEEDS THE 40TH TO 50TH PASSAGE LEVEL AND UNTIL THE PROPORTION OF CELLS HAVING ABNORMAL CHROMOSOMAL CHARACTERISTICS RISES ABOVE 25% OF THE TOTAL. FHT DIPLOID CELL LINE IS DERIVED FROM TISSUES OF FELINE EMBRYOS WHICH HAVE BEEN CULTURED AND SUBCULTURED UP TO THE 40TH PASSAGE LEVEL. THE HETEROPLOID CELL LINE IS SEPARATED FROM THE CULTURING MEDIUM AND INOCULATED WITH A VIRULENT STRAIN OF A VIRUS SO AS TO PROPAGATE THE VIRUS THEREON. THE PROPAGATED VIRUS STRAIN IS THEN FURTHER PASSAGED ON HETEROPLOID CELL LINES TO PRODUCE AN ATTENUATED ANTIGENIC MATERIAL THAT MAY BE USED IN A VACCINE. THE PROPAGATED VIRUS STRAIN MAY ALSO BE INACTIVATED WITH A SUITABLE INACTIVATING AGENT SO AS TO PRODUCE AN INACTIVATED ANTIGENIC MATERIAL THAT MAY BE USED IN A VACCINE.
Inventors
- 👤 S Smith
- 👤 Reilly K O
- 👤 J Prydie
Bibliographic Details
Claims Analysis
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1976-01-09 | 1976-07-10 | 1977-01-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-01-09 | 1980-07-10 | 1981-01-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-01-09 | 1984-07-10 | 1985-01-09 | $7700.00 | Window Closed / Lapsed |