US3708576A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel anti-inflammatory substances and production thereof
Assignee: Ono Pharmaceutical Co Ltd
•
Filing: 1970-05-08
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Granted: 1973-01-02
•
Expires: 1990-05-08
Abstract
Abstract Novel anti-inflammatory substances are provided by culturing a microorganism such as Aerobacter cloacae, Aerobacter aerogenes, Bacillus subtilis, Micrococus lysodeikticus or Pseudomonas aeruginosa in a nutrient medium and recovering the novel substance from the fermentation broth.
Inventors
- 👤 M Sugiura
- 👤 K Kano
- 👤 K Kimura
- 👤 H Oono
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 03:42
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/828; Y10S435/832; Y10S435/839; Y10S435/859; Y10S435/862; Y10S435/874; Y10S435/875
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
55 words
A process for producing the high molecular weight anti-inflammatory substance of claim 1 cultivating a microorganism belonging to Aerobacter cloacae, Aerobacter aerogenes, Bacillus subtilis, Micrococus lysodeikticus or Pseudomonas aeruginosa in a nutrient medium containing a carbon source, nitrogen source and inorganic salts and recovering the high molecular weight anti-inflammatory substance from the resulting fermentation broth.
Claim 2
Dependent Claim
31 words
The process as claimed in claim 2 wherein the anti-inflammatory substance is precipitated from a filtrate of the fermentation broth by the addition of ethanol in the presence of potassium chloride.
Claim 3
Dependent Claim
35 words
The process as claimed in claim 2 wherein a filtrate of the fermentation broth is concentrated, dialyzed to remove low molecular weight substances, and subjected to gel-filtration to obtain a fraction containing the anti-inflammatory substance.
Claim 4
Dependent Claim
49 words
The process as claimed in claim 3 wherein potassium chloride is added to the filtrate in an amount such that the potassium chloride concentration is 1-5 percent and then ethanol is added thereto in an amount such that the alcohol concentration is 40-80 percent, thus precipitating the anti-inflammatory substance.
References Cited (Backward Citations)
US2386994A
Cited
US2850427A
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US2895882A
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US3037915A
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US3031380A
Cited
US3394052A
Cited
US3431175A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-01-02 | 1976-07-03 | 1977-01-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-01-02 | 1980-07-03 | 1981-01-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-01-02 | 1984-07-03 | 1985-01-02 | $7700.00 | Window Closed / Lapsed |