Patent JPS5543421A successfully refreshed from Google Patents.
US3674861A
Expired / Lapsed
US
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Live attenuated marek{40 s disease virus vaccine for poultry
Assignee: Individual
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Filing: 1969-10-31
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Granted: 1972-07-04
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Expires: 1989-10-31
Abstract
Abstract Marek''s disease virus which causes a serious infection in poultry may be attenuated by serial passage in tissue culture. Passaging causes the loss of the A antigen after a certain number of passes while important protective antigens are retained. The virus may be attenuated in avian cells particularly those of the chick and duck, kidney cells and embryo fibroblasts being especially preferred. Usually about 20 to 40 passes are required. The live protective virus so obtained is cultivated further into a vaccine for parenteral administration.
Inventors
- 👤 Anthony Edward Churchill
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 13:10
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/245; A61K39/255; A61K2039/51; A61K2039/525; A61K2039/5254; A61K2039/55; A61K2039/552; C; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16311; C12N2710/16334; Y; Y10; Y10S; Y10S424/00; Y10S424/816
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
16 words
Process according to claim 1 in which said avian cells are chicken cells or duck cells.
Claim 2
Dependent Claim
13 words
Process according to claim 2 in which said avian cells are kidney cells.
Claim 3
Dependent Claim
12 words
Process according to claim 2 in which said avian cells are fibroblasts.
Claim 4
Dependent Claim
30 words
Process according to claim 1 in which the virus is passaged in one species of avian cell susceptible to tHe virus and then in another species of virus-susceptible avian cell.
Claim 5
Dependent Claim
28 words
Process according to claim 5 in which the virus is first passaged in chick embryo kidney cells and subsequently passaged in chick embryo fibroblasts or duck embryo fibroblasts.
Claim 6
Dependent Claim
20 words
Process according to claim 6 in which the number of passages in the fibroblasts exceeds that in the kidney cells.
Claim 7
Dependent Claim
19 words
Process according to claim 7 in which the number of passages in kidney cells is from 1 to 6.
Claim 8
Dependent Claim
16 words
Process according to claim 1 in which the total number of passages is at least 20.
Claim 9
Dependent Claim
15 words
Process according to claim 1 in which the number of passages is at least 25.
Claim 10
Dependent Claim
16 words
Process according to claim 1 in which the number of passages is from 31 to 39.
Claim 11
Dependent Claim
50 words
A live vaccine protective against Marek''s disease comprising as an antigenic component an effective protective amount of plaque-forming units per unit dose of an attenuated form of the virus of Marek''s disease substantially free of the A antigen, produced by the process of claim 1 or by further multiplication thereof.
Claim 12
Dependent Claim
22 words
A vaccine according to claim 12 containing from about 5 X 102 to about 7 X 103 plaque-forming units per unit dose.
Claim 13
Dependent Claim
39 words
A method of protecting poultry against Marek''s disease which comprises inoculating the chick embryo or the young chick with an effective protective unit dose of a live virus vaccine substantially free of A antigen as defined in claim 12.
Claim 14
Dependent Claim
31 words
A method according to claim 14 in which the effective protective unit dose of live virus vaccine substantially free of A antigen is given by subcutaneous, intramuscular, intranasal or intraocular injection.
Claim 15
Dependent Claim
20 words
A method according to claim 14 in which the live virus vaccine is injected into the leg of the chick.
Claim 16
Dependent Claim
17 words
A method according to claim 14 in which the live virus vaccine is given by intra-abdominal injection.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-07-04 | 1976-01-03 | 1976-07-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-07-04 | 1980-01-03 | 1980-07-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-07-04 | 1984-01-03 | 1984-07-04 | $7700.00 | Window Closed / Lapsed |