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Polyanionic compounds in culture media

Assignee: F Hoffmann La Roche AG
•
Filing: 1968-08-30
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Granted: 1972-06-20
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Expires: 1988-08-30
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Abstract

Abstract Sulfated polysaccharide, polyethylene sulfonic acid, polyvinyl sulfuric acid, polystyreme sulfonic acid, a sulfate ester of a polyethylene ether of an aliphatic alcohol having from 12 to 18 carbon atoms, monothioglycerol or sulfated naphthalene are combined with a bacterial culture medium. These compositions inhibit the antibacterial components of contaminated body fluids, thereby allowing the contaminant bacteria to grow freely.

Inventors

  • 👤 Thomas Cekoric Jr
  • 👤 George Evans
  • 👤 Ronald Searcy

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-27 22:35
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/045; Y; Y10; Y10S; Y10S435/00; Y10S435/80

Claims Analysis

Total Claims: 21
Claim 1
Independent Claim 31 words
The composition of claim 1 wherein there is present also a salt of polyanethol sulfonate in an amount not exceeding about 0.5 percent of the weight of the said culture medium.
Claim 2
Dependent Claim 12 words
The composition of claim 1 wherein ingredient (b) is a sulfated polysaccharide.
Claim 3
Dependent Claim 16 words
The composition of claim 1 wherein ingredient (b) is polyethylene sulfonic acid or a salt thereof.
Claim 4
Dependent Claim 16 words
The composition of claim 1 wherein ingredient (b) is polyvinyl sulfuric acid or a salt thereof.
Claim 5
Dependent Claim 16 words
The composition of claim 1 wherein ingredient (b) is polystyrene sulfonic acid or a salt thereof.
Claim 6
Dependent Claim 54 words
A composition for culturing microorganisms from body fluids containing antibacterial components which comprises (a) a culture medium and, based on the weight of said aqueous medium, from about 0.05 percent to about 2.0 percent of (b) a sulfate ester of a polyoxyethylene ether of an aliphatic alcohol having from 12 to 18 carbon atoms.
Claim 7
Dependent Claim 10 words
The composition of claim 1 wherein ingredient (b) is monothioglycerol.
Claim 8
Dependent Claim 12 words
The composition of claim 1 wherein ingredient (b) is a sulfonated napthalene.
Claim 9
Dependent Claim 20 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated polygalacturonic acid methyl ester methyl glycoside.
Claim 10
Dependent Claim 16 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated polymannuronic acid.
Claim 11
Dependent Claim 18 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated polygalacturonic acid methyl glycoside.
Claim 12
Dependent Claim 15 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated cycloheptaamylose.
Claim 13
Dependent Claim 15 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated cyclohexaamylose.
Claim 14
Dependent Claim 18 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated polyglucuronic acid methyl ester.
Claim 15
Dependent Claim 18 words
The composition of claim 3 wherein the sulfated polysaccharide is a salt of sulfated polymannuronic acid methyl glycoside.
Claim 16
Dependent Claim 12 words
The composition of claim 3 wherein chitosan polysulfuric acid ester is present.
Claim 17
Dependent Claim 10 words
The composition of claim 3 wherein dextran sulfate is present.
Claim 18
Dependent Claim 9 words
The composition of claim 3 wherein carrageenan is present.
Claim 19
Dependent Claim 14 words
The composition of claim 3 wherein a salt of polyanhydromannuronic acid sulfate is present.
Claim 20
Independent Claim 103 words
A method for producIng a medium for culturing microorganisms from body fluids containing antibacterial components which comprises adding to a culture medium from about 0.05 percent to about 2.0 percent by weight, based on the weight of said culture aqueous medium, of a compound selected from the group consisting of (1) a sulfated polysaccharide, (2) polyethylene sulfonic acid or a salt thereof, (3) polyvinyl sulfuric acid or a salt thereof, (4) polystyrene sulfonic acid or a salt thereof, (5) a sulfate ester of a polyoxyethylene ether of an aliphatic alcohol having from 12 to 18 carbon atoms, (6) monothioglycerol and (7)a sulfonated napthalene.
Claim 21
Independent Claim 109 words
A method for detecting certain infectious processes such as bacteremia and septicemia which comprises adding a body fluid containing antibacterial components to a culture medium which contains, based on the weight of said culture aqueous medium, from about 0.05 percent to about 2.0 percent by weight of a compound selected from the group consisting of (1) a sulfated polysaccharide, (2) polyethylene sulfonic acid or a salt thereof, (3) polyvinyl sulfuric acid or a salt thereof, (4) polystyrene sulfonic acid or a salt thereof, (5) a sulfate ester of a polyoxyethylene ether of an aliphatic alcohol having from 12 to 18 carbon atoms, (6) monothioglycerol and (7) a sulfonated naphthalene.

References Cited (Backward Citations)

US2596972A Cited
US2897193A Cited
US2976212A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1975-06-20 1975-12-20 1976-06-20 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1979-06-20 1979-12-20 1980-06-20 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1983-06-20 1983-12-20 1984-06-20 $7700.00 Window Closed / Lapsed

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