US3669838A
Expired / Lapsed
US
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Method of making antibiotics containing the aminocyclitol subunit
Assignee: University of Illinois at Urbana-Champaign
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Filing: 1970-03-17
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Granted: 1972-06-13
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Expires: 1990-03-17
Abstract
Abstract The method of making an antibiotic containing an aminocyclitol subunit. A microorganism mutant, incapable of biosynthesizing the aminocyclitol molecule but which molecule the unmutated microorganism biosynthesizes in the formation of an antibiotic when cultivated in a nutrient medium having no added aminocyclitol, is grown in the presence of an added aminocyclitol until substantial antibiotic activity is imparted to a culture medium, and the antibiotic is separated from the medium. Streptomyces fradiae ATCC 21401 mutant grown in the presence of added streptamine gives hybrimycins A1 and A2, and when grown in the presence of 2-epistreptamine gives hybrimycins B1 and B2. Streptomyces rimosus forma paromomycinus ATCC 21485 mutant grown in the presence of added streptamine gives hybrimycins C1 and C2. The hybrimycins have antibiotic activity.
Inventors
- 👤 Wayne Thomas Shier
- 👤 Kenneth L Rinehart Jr
- 👤 David Gottlieb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 08:54
CPC Classifications
C; C07; C07H; C07H15/00; C07H15/20; C07H15/22; C07H15/222; C07H15/226; C07H15/228; C07H15/232; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886; Y10S435/896; Y10S435/898; Y10S435/904
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
23 words
The method of claim 1 in which the mutant is incapable of biosynthesizing deoxystreptamine, and deoxystreptamine and the added aminocyclitol have dissimilar groups.
Claim 2
Independent Claim
151 words
The method of making an aminocyclitol-containing antibiotic which comprises: cultivating a mutant of (a) Streptomyces fradiae, (b) Streptomyces albogriseolus, (c) Streptomyces kanamyceticus, (d) Streptomyces rimosus forma paromomycinus, (e) Micromonospora echinospora, (f) Micromonospora purpurea, (g) Streptomyces tenebrarius, (h) Streptomyces hygroscopicus, (i) Streptomyces noboritoensis, (j) Streptomyces spectabilis, and (k) Streptomyces flavopersicus, in an aqueous medium containing a soluble carbohydrate, a source of assimilable nitrogen, essential mineral salts and an added aminocyclitol; said mutant being incapable of biosynthesizing the aminocyclitol molecule but which molecule the unmutated microorganism biosynthesizes in the formation of an antibiotic according to the correlation; Unmutated Microorganism Antibiotic (a) and (b) neomycin (c) kanamycin (d) paromomycin (e) and (f) gentamicin (g) nebramycin (h) and (i) hygromycin (j) and (k) spectinomycin when cultivated in a nutrient medium having no added aminocyclitol; continuing growing the mutant until substantial antibiotic activity is imparted to the culture medium; and, separating the antibiotic from the medium.
Claim 3
Independent Claim
60 words
The method of making an antibiotic containing an aminocyclitol subunit which comprises: cultivating the microorganism Streptomyces fradiae ATCC 21401 in an aqueous medium containing a soluble carbohydrate, a source of assimilable nitrogEn, essential mineral salts and an added aminocyclitol; continuing growing the microorganism until substantial antibiotic activity is imparted to the culture medium; and separating the antibiotic from the medium.
Claim 4
Dependent Claim
12 words
The method of claim 4 in which the added aminocyclitol is streptamine.
Claim 5
Dependent Claim
12 words
The method of claim 4 in which the added aminocyclitol is 2-epistreptamine.
Claim 6
Dependent Claim
19 words
The method of claim 5 in which the antibiotic produced is a mixture of hybrimycin A1 and hybrimycin A2.
Claim 7
Dependent Claim
19 words
The method of claim 6 in which the antibiotic produced is a mixture of hybrimycin B1 and hybrimycin B2.
Claim 8
Independent Claim
64 words
The method of making an antibiotic containing an aminocyclitol subunit which comprises: cultivating the microorganisms Streptomyces rimosus forma paromomycinus ATCC 21484 or 21485 in an aqueous medium containing a soluble carbohydrate, a source of assimilable nitrogen, essential mineral salts and an added aminocyclitol; continuing growing the microorganism until substantial antibiotic activity is imparted to the culture medium; and separating the antibiotic from the medium.
Claim 9
Dependent Claim
12 words
The method of claim 9 in which the added aminocyclitol is streptamine.
Claim 10
Independent Claim
54 words
The method of making an antibiotic containing an aminocyclitol subunit which comprises: cultivating the microorganism Streptomyces kanamyceticus ATCC 21486 in an aqueous medium containing a soluble carbohydrate, a source of assimilable nitrogen, essential mineral salts and an added aminocyclitol; and continuing growing the microorganism until substantial antibiotic activity is imparted to the culture medium.
Claim 11
Dependent Claim
12 words
The method of claim 1 in which the added aminocyclitol is 2-epistreptamine.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-06-13 | 1975-12-13 | 1976-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-06-13 | 1979-12-13 | 1980-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-06-13 | 1983-12-13 | 1984-06-13 | $7700.00 | Window Closed / Lapsed |