Patent US2766554A successfully refreshed from Google Patents.
US3663395A
Expired / Lapsed
US
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Cross-section illuminator for a continuous particle electrophoresis cell
Assignee: Beckman Coulter Inc
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Filing: 1969-12-17
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Granted: 1972-05-16
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Expires: 1989-12-17
Abstract
Abstract In a continuous particle electrophoresis cell, the present invention comprises means for illuminating a section of the electrolyte curtain with a narrow blade of light rather than with a broad beam which would flood the area of interest. Such blade of light illuminates the bands of sample particles only where they intersect the plane of light. By viewing the electrophoresis cell at a suitable angle, against a dark background, the crosssectional pattern of the particle band distribution becomes fully visible. This provides a valuable, practical tool for gauging and optimizing instrument performance.
Inventors
- 👤 Allen Strickler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:36
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721; G01N21/00; G01N21/17; G01N21/41; G01N21/45
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
18 words
In a continuous particle electrophoresis apparatus according to claim 1, the improvement wherein said radiation is visible light.
Claim 2
Dependent Claim
66 words
In a continuous particle electrophoresis apparatus according to claim 2, the improvement wherein said means for observing said curtain cross-section comprises: a transparent window in one of said plates of said apparatus, the other of said plates being transparent; and a dark background positioned on the outside of said other plate whereby said curtain cross-section may be viewed through said transparent window against said dark background.
Claim 3
Dependent Claim
23 words
In a continuous particle electrophoresis apparatus according to claim 1, the improvement wherein said radiation is in the ultraviolet region of the spectrum.
Claim 4
Dependent Claim
26 words
In a continuous particle electrophoresis apparatus according to claim 1, the improvement wherein said blade of light is thin compared with the thickness of said curtain.
Claim 5
Dependent Claim
50 words
In a continuous particle electrophoresis apparatus according to claim 1, the improvement wherein said means for illuminating a cross-section of said curtain comprises: a line source of light lying in a plane parallel to said curtain; and means for imaging said light source substantially in the plane of said curtain.
Claim 6
Dependent Claim
59 words
In a continuous particle electrophoresis apparatus according to claim 6, the improvement wherein the axial plane defined by said line source and its image at said curtain is inclined to the plane of said curtain, and wherein said means for observing said curtain cross-section permits observation of said curtain cross-section at an angle with respect to said optic axis.
Claim 7
Dependent Claim
43 words
In a continuous particle electrophoresis apparatus according to claim 1, the improvement wherein said blade of radiation intersects said curtain along a line perpendicular to the direction of said flow, and has an angle of incidence on said curtain of less than 90*.
Claim 8
Dependent Claim
53 words
In a continuous particle electrophoresis apparatus according to claim 1, the improvement wherein said means for illuminating a cross-section of said curtain comprises: first and second spaced,co-planar blades forming a straight-edged slit therebetween; means for illuminating said slit; and means for focusing an image of said slit in the plane of said curtain.
Claim 9
Dependent Claim
61 words
In a continuous particle electrophoresis apparatus having a thin curtain of liquid carrier medium which flows in an electrophoresis space between a pair of spaced plates, means for illuminating a cross-section of the liquid curtain comprising: a line source of visible light; and means for imaging said line source in the plane of said curtain perpendicular to the curtain flow direction.
Claim 10
Dependent Claim
39 words
In a continuous particle electrophoresis apparatus according to claim 10, wherein said line source and said focusing means illuminate said cross-section of said curtain with a blade of light which is thin compared with the thickness of said curtain.
Claim 11
Dependent Claim
53 words
In a continuous particle electrophoresis apparatus having a thin curtain of liquid carrier medium flowing in an electrophoresis space between a pair of spaced plates, a method for observing the performance of said apparatus comprising: illuminating a cross-section of said curtain transverse to the curtain flow direction with a narrow blade of radiation.
Claim 12
Dependent Claim
18 words
In a continuous particle electrophoresis apparatus according to claim 12, the method wherein said radiation is visible light.
Claim 13
Dependent Claim
22 words
In a continuous particle electrophoresis apparatus according to claim 13, the method further comprising: viewing said illuminated cross-section against a dark background.
Claim 14
Dependent Claim
47 words
In a continuous particle electrophoresis apparatus according to claim 12, the method wherein said step of illuminating a cross-section of said curtain comprises the step of: illuminating a cross-section of said curtain with a blade of light which is thin compared with the thickness of said curtain.
Claim 15
Dependent Claim
41 words
In a continuous particle electrophoresis apparatus according to claim 12, the method wherein said step of illuminating a cross-section of said curtain comprises the step of: illuminating said cross-section at an angle which is inclined to the plane of said curtain.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-05-16 | 1975-11-15 | 1976-05-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-05-16 | 1979-11-15 | 1980-05-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-05-16 | 1983-11-15 | 1984-05-16 | $7700.00 | Window Closed / Lapsed |