US3655570A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detergent containing alkali protease
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1969-02-03
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Granted: 1972-04-11
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Expires: 1989-02-03
Abstract
Abstract Alkaline protease if produced by cultivating an alkaline protease-producing microorganism from the genus Fusarium or Giberella in a proper growth supporting medium and the alkaline protease is recovered from the medium. The alkaline protease degrades protein under conditions of high pH and thus is useful in the formulation of detergent and other cleanser compositions.
Inventors
- 👤 Masao Isono
- 👤 Katsumi Tomoda
- 👤 Kouichi Miyata
- 👤 Kazutaka Maejima
- 👤 Keisuke Tsubaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:35
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/58; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
10 words
molecular weight of about 2.65 X 104 (by Archibald''s method,
Claim 2
Dependent Claim
33 words
A composition as in claim 1, wherein the amount of the said alkali protease is from about 5 to about 10,000 PU per gram of the final product by the modified Kunitz'' method.
Claim 3
Dependent Claim
20 words
A composition as in claim 1, wherein the said surfactant is selected from an anionic surfactant and a nonionic surfactant.
Claim 4
Dependent Claim
20 words
elementary analysis about 46.72 percent by weight carbon, about 6.59 percent by weight hydrogen and 15.3 percent by weight nitrogen,
Claim 5
Dependent Claim
31 words
A composition as in claim 3, wherein the said anionic surfactant is from the group of a fatty acid salt, alkyl sulfate, olefine sulfate, alkyl sulfonate, olefine sulfonate, and alkylaryl sulfonate.
Claim 6
Dependent Claim
21 words
maximum absorption, in its ultraviolet absorption spectrum at the wavelength of 275 to 280 Mu , as indicated in FIG. 1,
Claim 7
Dependent Claim
34 words
infrared absorption spectrum as shown in FIG. 2, wherein the significant absorption bands in microns are as follows: 3.0 (strong), 3.38 (medium), 6.05 (strong), 6.55 (strong), 6.88 (weak), 7.15 (medium), 8.12 (medium), 9.30 (weak),
Claim 8
Dependent Claim
32 words
A composition as in claim 3, wherein the said nonionic surfactant is from the group of polyoxyethylene-alkyl ether, polyoxyethylene alkyl phenol ether, polyoxy-ethylene alkyl ester, polyhydric alcohol alkyl ester and sugar ester.
Claim 9
Dependent Claim
5 words
isoelectric point about pH 11,
Claim 10
Dependent Claim
30 words
optimal activity at a pH from 8 to 12 as indicated in FIG. 3, and at a temperature from about 40* to about 50* C. as indicated in FIG. 4,
Claim 11
Dependent Claim
15 words
stable in the pH range from 5 to 8 in 1 hour-incubation at 37* C.,
Claim 12
Dependent Claim
22 words
20 and 80 percent loss of activity upon being heated at 65* and 70* C., respectively, in 10 minute-incubation at pH 5.0.
References Cited (Backward Citations)
US3451935A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-04-11 | 1975-10-11 | 1976-04-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-04-11 | 1979-10-11 | 1980-04-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-04-11 | 1983-10-11 | 1984-04-11 | $7700.00 | Window Closed / Lapsed |