US3655513A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of protease
Assignee: Bayer Corp
•
Filing: 1971-01-25
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Granted: 1972-04-11
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Expires: 1991-01-25
Abstract
Abstract Proteases can be isolated and separated from impurities, such as colored bodies, sugars, polysaccharides and minerals, by mixing a protease solution containing impurities with a heteropoly acid to form a precipitate with the protease. The resulting precipitate is then separated from the remaining solution and dried. Useful heteropoly acids are phosphotungstic acid, arsenotungstic acid, silicotungstic acid, borotungstic acid, phosphomolybdic acid, arsenomolybdic acid, silicomolybdic acid, boromolybdic acid, phosphovanadic acid, arsenomolybdic acid, silicovanadic acid, borovanadic acid and mixtures and combinations thereof.
Inventors
- 👤 Moshe Sternberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 12:10
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
28 words
A process according to claim 1 wherein the concentration of the heteropoly acid in the protease solution is from about 0.05 percent to about 1 percent (weight/volume basis).
Claim 2
Dependent Claim
36 words
A process according to claim 1 wherein the concentration of the heteropoly acid in the protease solution is about 0.5 percent (weight/volume basis) and the pH of the protease solution from about 4.5 to about 5.0.
Claim 3
Dependent Claim
13 words
A process according to claim 1 wherein the heteropoly acid is phosphotungstic acid.
Claim 4
Dependent Claim
13 words
A process according to claim 1 wherein the heteropoly acid is phosphomolybdic acid.
Claim 5
Dependent Claim
13 words
A process according to claim 1 wherein the heteropoly acid is borotungstic acid.
Claim 6
Dependent Claim
34 words
A process according to claim 1 wherein the protease solution contains calcium or zinc salts in a molar concentration of from about 0.001 to about 0.1 at the time the heteropoly acid is added.
References Cited (Backward Citations)
US3483085A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-04-11 | 1975-10-11 | 1976-04-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-04-11 | 1979-10-11 | 1980-04-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-04-11 | 1983-10-11 | 1984-04-11 | $7700.00 | Window Closed / Lapsed |