US3645849A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stability of enzymes
Assignee: ETC Ltd A FL LP
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Filing: 1969-04-17
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Granted: 1972-02-29
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Expires: 1989-04-17
Abstract
Abstract Enzymes in an aqueous medium are cooled to 50* F. or below and then subjected to microwave energy under controlled conditions whereby the stability of the enzymes against deterioration is materially increased.
Inventors
- 👤 Oscar S Gray
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:06
CPC Classifications
C; C12; C12N; C12N13/00; A; A61; A61K; A61K41/00; A61K41/10; A61K41/13
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
22 words
The method of claim 1 wherein said microwave energy has a frequency of from about 1,000 to about 5,000 megacycles per second.
Claim 2
Dependent Claim
22 words
The method of claim 2 wherein said microwave energy has a frequency of from about 2,000 to about 3,000 megacycles per second.
Claim 3
Dependent Claim
22 words
The method of claim 3 wherein said microwave energy has a frequency of from about 2,400 to about 2,500 megacycles per second.
Claim 4
Dependent Claim
30 words
The method of claim 1 wherein after exposure to the microwave energy the treated enzyme in the container is continued to be subjected to the action of said coolant gas.
Claim 5
Dependent Claim
30 words
The method of claim 3 wherein after exposure to the microwave energy the treated enzyme in the container is continued to be subjected to the action of said coolant gas.
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein said enzyme in aqueous medium is at least one of those in blood serum.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein the enzyme in aqueous medium is blood serum.
Claim 8
Dependent Claim
28 words
The method of claim 8 wherein the blood serum is cooled to a temperature of from about 35* to about 45* F. before exposure to said microwave energy.
Claim 9
Dependent Claim
22 words
The method of claim 8 wherein the microwave energy has a frequency of from about 2,000 to about 3,000 megacycles per second.
Claim 10
Dependent Claim
25 words
The method of claim 8 wherein the blood serum does not exceed a temperature of about 125* F. during said exposure to said microwave energy.
Claim 11
Dependent Claim
28 words
The method of claim 8 wherein, after discontinuing the exposure of said blood serum to said microwave energy, said blood serum is cooled to below about 50* F.
Claim 12
Dependent Claim
66 words
The method of claim 9 wherein the microwave energy has a frequency of from about 2,000 to about 3,000 megacycles per second; wherein the blood serum does not exceed a temperature of about 125* F. during said exposure to said microwave energy; and wherein, after discontinuing the exposure of said blood serum to said microwave energy, said blood serum is cooled to below about 50* F.
Claim 13
Dependent Claim
26 words
The methOd of claim 1 wherein substantially all of the major microwave-permeable walls of said container are presented directly to said microwave energy during said exposure.
Claim 14
Dependent Claim
26 words
The method of claim 3 wherein substantially all of the major microwave-permeable walls of said container are presented directly to said microwave energy during said exposure.
Claim 15
Dependent Claim
26 words
The method of claim 5 wherein substantially all of the major microwave-permeable walls of said container are presented directly to said microwave energy during said exposure.
Claim 16
Dependent Claim
26 words
The method of claim 8 wherein substantially all of the major microwave-permeable walls of said container are presented directly to said microwave energy during said exposure.
Claim 17
Dependent Claim
26 words
The method of claim 10 wherein substantially all of the major microwave-permeable walls of said container are presented directly to said microwave energy during said exposure.
Claim 18
Dependent Claim
26 words
The method of claim 13 wherein substantially all of the major microwave-permeable walls of said container are presented directly to said microwave energy during said exposure.
Claim 19
Dependent Claim
30 words
Blood serum treated in accordance with the method of claim 8 and in which enzymes thereof possess improved stability against deterioration as compared to similar blood serum not so treated.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-02-28 | 1975-08-30 | 1976-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-02-28 | 1979-08-30 | 1980-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-02-28 | 1983-08-30 | 1984-02-29 | $7700.00 | Window Closed / Lapsed |