US3645698A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Sampling apparatus
Assignee: Beckman Coulter Inc
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Filing: 1969-10-06
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Granted: 1972-02-29
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Expires: 1989-10-06
Abstract
Abstract An apparatus for monitoring the progress of a peptide synthesis process being carried out in a closed reaction vessel including a slidable member having a plurality of pockets for trapping small aliquots of peptide-resin cooperating with the interior of the reaction vessel for selectively removing samples of peptide-resin without exposing the interior of the reaction vessel to the surrounding environment.
Inventors
- 👤 Leslie D Holybee
- 👤 Henry Faigh
- 👤 Robert B Samuels
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 14:46
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/02; G01N1/10; G01N1/20; G01N1/2035; B; B01; B01J; B01J19/00; B01J19/0046; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00281; B01J2219/00286; B01J2219/00457; B01J2219/00459; B01J2219/00466; B01J2219/00583; B01J2219/0059; B01J2219/00596; B01J2219/0068; B01J2219/00698; B01J2219/00718; B01J2219/0072; B01J2219/00725; C; C40; C40B; C40B40/00; C40B40/04; C40B40/10; C40B60/00; C40B60/14
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
196 words
An apparatus for sequentially removing and retaining a plurality of samples of peptide-resin from a closed reaction vessel having a small aperture in a wall thereof to monitor the progress of a peptide-synthesis process comprising: a tubular housing having a predetermined inner diameter; means for fixedly securing said tubular housing to the outer wall of the reaction vessel about said aperture; a slidable member slidably disposed within said tubular housing for communicating with the interior of said reaction vessel through the aperture; and a plurality of discs secured to and spaced apart predetermined and equal distances along said slidable member to form a plurality of pockets for trapping and removing samples of peptide-resin from the interior of the reaction vessel, each of said discs having a diameter substantially equal to the inner diameter of said tubular housing; and said tubular housing extending outside of said reaction vessel for an axial distance which is greater than the distance between the first and last of said discs spaced along said slidable member whereby said samples in said pockets may be retained inside said tubular housing outside of said reaction vessel until all of said samples have been removed.
Claim 2
Dependent Claim
33 words
Apparatus as defined in claim 2 comprising in addition means coupled to one end of said slidable member for moving said member in a stepwise fashion to sequentially sample the peptide synthesis process.
Claim 3
Dependent Claim
41 words
Apparatus as defined in claim 2 wherein each of said discs includes a flexible lip seal about the outer edge thereof to provide a liquid tight seal with the inner wall of said tubular housing as the slidable member travels therethrough.
Claim 4
Independent Claim
191 words
An apparatus for sequentially securing samples of peptide-resin to monitor the course of a peptide synthesis process comprising: a closed reaction vessel within which the synthesis process is performed, said reaction having first and second separate apertures for the inlet and outlet of materials, each of said apertures being provided with closure means, and a third aperture in a wall thereof; an external tubular housing secured to an outer wall of said reaction vessel about said third aperture; a movable member slidably disposed within said tubular housing and communicating with the interior of said reaction vessel through said third aperture, said movable member being slidable into and out of said reaction vessel and including a plurality of pocket means for selectively and sequentially trapping and removing predetermined size aliquots of peptide-resin from the interior of said reaction vessel; and the length of said tubular housing extending outside of said reaction vessel being greater than the length of That portion of said movable member on which said plurality of pocket means are disposed whereby said samples in said pocket means may be retained in said tubular housing outside of said reaction vessel.
Claim 5
Dependent Claim
39 words
Apparatus as defined in claim 5 and comprising in addition means coupled to one end of said movable member for automatically moving said member in a stepwise fashion to sequentially sample the peptide synthesis process during the progress thereof.
Claim 6
Dependent Claim
55 words
Apparatus as claimed in claim 5 wherein said means for trapping predetermined size aliquots of peptide-resin comprises a plurality of discs secured to said movable member and spaced apart from each other to form a plurality of pockets for trapping and removing predetermined size aliquots of peptide resin from the interior of the reaction vessel.
Claim 7
Dependent Claim
107 words
Apparatus as claimed in claim 7 wherein each of said discs has a generally circular geometrical configuration, said movable member comprises a shaft having a circular cross section, and each pocket is bounded by two successive discs and comprises a hollow cylinder having an inner diameter equal to the diameter of said circular cross sectional shaft, an outer diameter equal to the diameter of said discs, and a height equal to the distance between two adjacent discs, and wherein the length of said tubular housing extending outside of said reaction vessel is greater than the distance between the first and last discs secured to said movable member.
Claim 8
Dependent Claim
57 words
Apparatus as claimed in claim 8 wherein said tubular housing includes a small aperture at one end thereof with which each pocket may be successively brought into registration and through which a rinsing solution flows to simultaneously remove the retained sample of peptide-resin from the pocket and wash out the pocket preparing it to receive another sample.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-02-28 | 1975-08-30 | 1976-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-02-28 | 1979-08-30 | 1980-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-02-28 | 1983-08-30 | 1984-02-29 | $7700.00 | Window Closed / Lapsed |