US3645689A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for analyzing proteins
Assignee: Pfizer Health AB
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Filing: 1970-04-09
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Granted: 1972-02-29
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Expires: 1990-04-09
Abstract
Abstract A method and an apparatus for determining the sequence of the amino acids in a protein. The protein is applied to the surface of a solid support arranged in a reactor. A vaporized coupling agent is supplied to the protein thus forming a protein derivative. The protein derivative is split at the first peptide binding by a supplied vaporized splitting agent and the amino acid derivative split off is transferred to an absorber. By raising the temperature of the absorber the amino acid derivative is then transferred to an analyzer, e.g., a gas chromatograph.
Inventors
- 👤 John A Sjoquist
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 20:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6818; G01N33/6824
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
42 words
Method according to claim 1, characterized in, that the protein applied to the solid support is treated with a blocking means which blocks reactive groups of the protein molecule, and thus prevents undesired side reactions of the protein and the splitting agent.
Claim 2
Dependent Claim
25 words
Method according to claim 1, characterized in, that the protein is adsorbed on the surface of a solid support, consisting of massive granulates without micropores.
Claim 3
Dependent Claim
26 words
Method according to claim 1, characterized in, that the coupling agent and the blocking agent supplied to the protein are mixed with an inert carrier gas.
Claim 4
Dependent Claim
15 words
Method according to claim 1, characterized in, that the coupling agent consists of a cyanate.
Claim 5
Dependent Claim
15 words
Method according to claim 1, characterized in, that the coupling agent consists of an isothiocyanate.
Claim 6
Dependent Claim
17 words
Method according to claim 1, characterized in, that the blocking agent consists of a silicon containing compound.
Claim 7
Dependent Claim
42 words
Method according to claim 1, characterized in, that after the splitting the adsorbed amino acid derivative is made to react with a vapor pressure increasing agent in order to facilitate the separation of the derivative from the remaining part of the protein.
Claim 8
Dependent Claim
18 words
Method according to claim 8, characterized in, that the vapor pressure increasing agent consists of a silicon-containing compound.
Claim 9
Dependent Claim
48 words
Method according to claim 9, characterized in, that the silicon containing groups are removed from the remaining part of the protein by a hydrolysis of vaporized water when the volatile amino acid derivative has been removed from the remaining part of the protein adsorbed on the solid support.
Claim 10
Dependent Claim
30 words
Method according to claim 3, characterized in, that the amino acid derivative is removed from the remaining part of the protein adsorbed on the solid support by raising the temperature.
Claim 11
Dependent Claim
30 words
Method according to claim 3, characterized in, that the amino acid derivative is removed from the remaining part of the protein adsorbed on the solid support by lowering the pressure.
Claim 12
Dependent Claim
34 words
Method according to claim 3, characterized in, that the amino acid derivative is removed from the remaining part of the protein adsorbed on the solid support by raising the temperature and lowering the pressure.
Claim 13
Dependent Claim
24 words
Method according to claim 1, characterized in, that the removal of the amino acid derivative is preceded by a removal of undesired side products.
Claim 14
Dependent Claim
28 words
Method according to claim 1, characterized in, that the amino acid derivative is transferred to a cooled absorber and by heating of the absorber transferred to an analyzer.
Claim 15
Dependent Claim
152 words
Apparatus for determining the sequence of the amino acids in a protein characterized in, that the apparatus comprises a reactor, including a solid support on which the protein is adsorbed, means for generating a flow of inert carrier gas, means for supplying this gas through the reactor, means for mixing the gas with a coupling agent in gas phase and for mixing the carrier gas with other reagents in gas phase, e.g., a blocking agent, the apparatus further comprising an absorber in which the volatile amino acid derivatives formed in the reactor are absorbed, means for heating the reactor in order to facilitate a transferring oF the amino acid derivative from the reactor to the absorber, an analyzer for identifying the amino acid comprised in the amino acid derivative and, means for heating the absorber so as to facilitate the transferring of the amino acid derivatives from the absorber to the analyzer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-02-28 | 1975-08-30 | 1976-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-02-28 | 1979-08-30 | 1980-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-02-28 | 1983-08-30 | 1984-02-29 | $7700.00 | Window Closed / Lapsed |