US3637463A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of oxytetracycline using streptomyces alboflavus (atcc 15388)
Assignee: ANGUS BIOTECH Inc A CORP OF DE
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Filing: 1967-08-16
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Granted: 1972-01-25
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Expires: 1987-08-16
Abstract
Abstract A fermentative process for the production of oxytetracycline which comprises fermenting a nutrient broth under aerobic conditions at a temperature between 22 DEG and 30 DEG C. by a mutant of Streptomyces alboflavus (Waksmann & Curtis), and recovering accumulated oxytetracycline from the fermented broth.
Inventors
- 👤 Ivan Villax
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 16:12
CPC Classifications
C; C12; C12P; C12P29/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
24 words
A process according to claim 1, wherein the fermentation is submerged and the nutrient medium contains assimilable carbon and nitrogen sources and mineral salts.
Claim 2
Dependent Claim
93 words
A process according to claim 1, wherein the fermented broth is acidified to a pH of 1 to 2 with an acid, the resultant medium is filtered, the pH of the filtrate is increased with aqueous ammonium to pH 5 to 6, N'', N''-dibenzylethylenediamine diacetate is added to a total concentration of from about one-tenth up to about one-fifth by weight of the oxytetracycline in the medium, the pH of the medium is adjusted to 8.5 to 9 with aqueous ammonium, and the oxytetracycline is recovered in the form of N, N''-dibenzylethylenediamine complex.
Claim 3
Dependent Claim
24 words
A process according to claim 3, wherein the nutrient medium contains calcium ions and the oxytetracycline is recovered in the form of N,N''-dibenzylethylenediamine-calcium complex.
Claim 4
Dependent Claim
32 words
A process according to claim 1, wherein N,N''-dibenzylethylenediamine diacetate is added portionwise to the nutrient broth during the fermentation, the amount added being from about 100 to about 3,000 milligrams/liter of medium.
References Cited (Backward Citations)
US2516080A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-01-24 | 1975-07-26 | 1976-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-01-24 | 1979-07-26 | 1980-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-01-24 | 1983-07-26 | 1984-01-25 | $7700.00 | Window Closed / Lapsed |