Method of preparing hemolysates for hemoglobin and other types of electrophoresis using chelating agents
Abstract
Abstract THE METHOD OF PREPARING A HEMOLYSATE INVOLVES THE LIBERATION OR SEPARATION OF THE HEMOGLOBIN FROM THE RED BLOOD CORPUSCLES OR CELLS BY SUBJECTING THE SOLUTION IN WHICH THE RED BLOOD CORPUSCLES ARE SUSPENDED TO A CHELATING AGENT DISSOLVED IN WATER WHICH LYSES THE CELLS. AS A RESULT THEREOF THE HEMOGLOBIN APPEARS IN THE SOLUTION COMBINED WITH THE CHELATING COMPOUND TO FORM CHELATING MOLECULES. THE LYSING SOLUTION I COMMONLY USE FOR THE RED BLOOD CELLS IS A DILUTE SOLUTION OF (ETHYLENEDINITRILO)~ TETRAAACETIC ACID TETRASODIUM SALT (IND THE RANGE OF .052.0%) IN WATER. THIS SOLUTION IS ADDED DIRECTLY TO THE RED BLOOD CELLS WHICH WERE PREVIOUSLY WASHED IN A .85% SALINE SOLUTION. THE RESULTING HEMOLYSTE IS READY FOR HEMOGLOBIN OR OTHER TYPES OF ELECTROPHORESIS (ISOENZYMES, ETC.) AFTER BEING THOROUGHLY MIXED.
Inventors
- 👤 Tipton L Golias
Bibliographic Details
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