US3632483A
Expired / Lapsed
US
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Electrode sensitive to choline and its esters and methods using said electrode
Assignee: Corning Glass Works
•
Filing: 1970-06-15
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Granted: 1972-01-04
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Expires: 1990-06-15
Abstract
Abstract An electrode for measuring the concentration of cations of choline and its esters in an aqueous solution wherein the sensing portion is a liquid organic phase containing a substituted tetraphenylborate dissolved in a suitable organic solvent.
Inventors
- 👤 George Baum
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 06:15
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/333; G01N27/3335; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
14 words
The electrode of claim 1, wherein said material is dissolved in a nitroaromatic solvent.
Claim 2
Dependent Claim
46 words
The electrode of claim 2, wherein said solvent has the formula wherein R is a member selected from the group consisting of hydrogen, alkyl containing 1-14 carbon atoms, lower alkoxy and alkylcarboxy, and R'' is a member selected from the group consisting of hydrogen and methyl.
Claim 3
Dependent Claim
13 words
The electrode of claim 3, wherein said ion exchange material is choline tetrakis(p-chlorophenyl)borate.
Claim 4
Dependent Claim
13 words
The electrode of claim 3, wherein said ion exchange material is acetylcholine tetrakis(p-chlorophenyl)borate.
Claim 5
Dependent Claim
10 words
The electrode of claim 3 wherein said solvent is 1,2-dimethyl-4-nitrobenzene.
Claim 6
Dependent Claim
34 words
The electrode of claim 2 wherein said means includes a container for said liquid organic phase having an opening at a portion and an organophilic-hydrophobic porous membrane disposed in covering relationship across said opening.
Claim 7
Dependent Claim
23 words
The electrode of claim 7, wherein said membrane consists of a ceramic material coated wiTh a treating agent to impart the organophilic-hydrophobic property.
Claim 8
Dependent Claim
19 words
The electrode of claim 7 wherein a dialysis membrane is positioned in front of and across said porous membrane.
Claim 9
Dependent Claim
14 words
The electrode of claim 9 wherein said dialysis membrane consists essentially of porous cellophane.
Claim 10
Independent Claim
84 words
A method of measuring the concentration of choline and its esters in an aqueous solution comprising the steps of a. providing a liquid organic ion exchange electrode wherein the liquid organic phase contains an ion exchange material having the formula ((CH3)3NCH2CH2OR) (B(C6H4X)4) wherein X is chlorine or phenoxy, R is hydrogen or and R'' is lower alkyl or phenyl, b. subjecting the aqueous solution to said liquid organic ion exchange electrode and a reference electrode, and c. measuring the potential developed between the electrodes.
Claim 11
Dependent Claim
11 words
The method of claim 11 wherein said aqueous solution contains acetylcholine.
Claim 12
Independent Claim
109 words
A method of determining the activity of an esterase in an aqueous solution containing acetylcholine and the esterase comprising the steps of a. providing a liquid organic ion exchange electrode wherein the liquid organic phase consists essentially of a nitroaromatic solvent and 0.5-10 percent by weight of an ion exchange material having the formula ((CH3)3NCH2CH2OR) (B(C6H4X)4) wherein X is chlorine or phenoxy, R is hydrogen or and R'' is lower alkyl or phenyl, b. subjecting the aqueous solution to said liquid organic ion exchange electrode and a reference electrode, c. measuring the potential developed between the electrodes, and d. calculating the rate of loss of acetylcholine by enzymatic hydrolysis.
Claim 13
Dependent Claim
10 words
The method of claim 13, wherein said esterase is cholinesterase.
Claim 14
Dependent Claim
10 words
The method of claim 13, wherein said esterase is acetylcholinesterase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-01-03 | 1975-07-05 | 1976-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-01-03 | 1979-07-05 | 1980-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-01-03 | 1983-07-05 | 1984-01-04 | $7700.00 | Window Closed / Lapsed |