US3630680A
Expired / Lapsed
US
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Diagnostic agents for the detection of urobilinogen materials in body fluids
Assignee: Roche Diagnostics GmbH
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Filing: 1968-10-18
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Granted: 1971-12-28
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Expires: 1988-10-18
Abstract
Abstract Diagnostic agents for use in the detection of urobilinogen materials in body fluids such as urine, the agents comprising at least one strong acid and at least one compound which reacts with urobilinogen materials present in the body fluids to provide analytically measurable dyestuffs wherein said compound has the formula: IN WHICH R1 and R2 which may be the same or different, each represent halogen, cyano, nitroso, carbalkoxy, carbamido, hydroxy, acyloxy, alkoxy, straight-chained and cyclic, saturated and unsaturated secondary and tertiary amino, or R1 and R2 together represent oxygen, sulfur, sulfonyl, alkylthionium, imino, alkylimino, substituted or unsubstituted arylimino or open-chained, alicyclic or heterocyclic dialkylimino, R2 can also represent the residue of secondary straight or branched, openchained or mono- or polycyclic alkyl or aralkyl and wherein one of R1 and R2 can also represent hydrogen, R3 is hydrogen or alkyl, and n and m which may be the same or different each have a value of 0, 1, 2 or 3.
Inventors
- 👤 Walter Rittersdorf
- 👤 Peter Rieckmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
126 words
Diagnostic agent according to claim 1 wherein said compound which reacts with urobilinogen bodies to provide an analytically measurable dyestuff has the formula: wherein R1 represents a member selected from the group consisting of hydrogen, halogen, cyano, nitroso, carbalkoxy, carbamido, hydroxy, acyloxy, alkoxy and amine radicals having at least one aliphatic hydrocarbon substituent and, if there are two aliphatic hydrocarbon substituents they may, together with the amine nitrogen, represent an N-aliphatic heterocycle, R2 represents a member selected from the group consisting of secondary straight-chained alkyl, branched-chained alkyl, monocyclic alkyl, polycyclic alkyl and aralkyl, R3 is a member selected from the group consisting of hydrogen and alkyl and n is 0, 1, 2 and 3, with the proviso that R1 is hydrogen only when n is 0.
Claim 2
Dependent Claim
41 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution containing 0.001-1 percent of a compound (I) as defined in claim 1 and 15-30 percent of a strong solid acid.
Claim 3
Dependent Claim
36 words
Diagnostic agent according to claim 1 wherein said strong acid is a member selected from the group consisting of sulfosalicyclic acid, p-toluene-sulfonic acid, sulfuric acid, glutamic acid, o-phosphoric acid, oxalic acid, hydrochloric acid and maleic acid.
Claim 4
Dependent Claim
38 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution having the following composition: p-bis-( Beta -diethylaminoethyl)-aminobenzaldehyde 0.2 g. oxalic acid 20.0 g. methanol ad 100.0 ml.
Claim 5
Dependent Claim
46 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution having the following composition: p-bis-( Beta -cyanoethyl)-aminobenzaldehyde 0.05 g. oxalic acid 20.0 g. polyvinyl alcohol 3.0 g. water 30.0 ml. methanol ad 100.0 ml. c
Claim 6
Dependent Claim
52 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution having the following composition: 0.05 and 0.2 parts of a member selected from the group consisting of p-N-(N''-methyl-piperazino)-benzaldehyde and p-dimethylaminobenzaldehyde and 20 parts of oxalic acid in 100 parts of methanol.
Claim 7
Dependent Claim
59 words
Diagnostic agent according to claim 1 wherein said carrier is a polyvinylchloride film having applied thereon a layer of the following composition: p-bis-( Beta -cyanoethyl)-aminobenzaldehyde 0.75 g. syrupy orthophosphoric acid 5.00 g. colloidal silicic acid (''''Aerosil'''') 6.00 g. organic sodium sulfonate (rapid wetting agent) 2.00 g. polyvinyl butyracetal (''''Mowital'''') 10.00 g. polyethylene glycol 6000 10.00 g. methanol 100.00 ml.
Claim 8
Dependent Claim
20 words
Diagnostic agent according to claim 1 wherein said carrier is a liquid containing p-N-morpholinobenzaldehyde in solution in concentrated hydrochloric acid.
Claim 9
Dependent Claim
44 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution having the following composition: 0.5 parts p-bis-(diethylaminoethyl)-aminobenzaldehyde bis-hydrogen oxalate, 30 parts maleic acid and 0.5 parts polyethylene glycol 1,000-20,000 in 100 parts methanol.
Claim 10
Dependent Claim
36 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution having the following composition: p-(cyclohexylamino)-benzaldehyde 0.05 g. oxalic acid 20.0 g. methanol ad 100.0 ml.
Claim 11
Dependent Claim
40 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution having the following composition: p-(sec.-butylamino)-benzaldehyde 0.05 g. potassium bisulfate 15.0 g. polyethylene glycol 5.0 g. water ad 100.0 ml.
Claim 12
Dependent Claim
46 words
Diagnostic agent according to claim 1 wherein said carrier is a polyvinylchloride film having applied thereon a layer of the following composition: c p-(cyclohexylamino)-benzaldehyde 0.05 g. colloidal silicic acid (''''Aerosil'''') 1.00 g. oxalic acid 10.00 g. polyvinyl butyracetal (''''Mowital'''') 0.5 g. polyethylene glycol 6000 0.5 g.
Claim 13
Dependent Claim
42 words
Diagnostic agent as claimed in claim 1 wherein said compound has the formula wherein R2 is a secondary alkyl group which can be straight-chained, branched or cyclic and wherein R3 is a member selected from the group consisting of hydrogen and alkyl.
Claim 14
Dependent Claim
17 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface.
Claim 15
Dependent Claim
55 words
A method for carrying out an analytical test for the presence of urobilinogen bodies in biological fluids which comprises applying to the wettable surface of a diagnostic agent according to claim 15 a small amount of the fluid to be tested and examining said paper for visual evidence of the presence of said urobilinogen bodies.
Claim 16
Dependent Claim
12 words
Diagnostic agent according to claim 1 wherein said carrier is a foil.
Claim 17
Dependent Claim
55 words
A method for carrying out an analytical test for the presence of urobilinogen bodies in biological fluids which comprises applying to the coated surface of a diagnostic agent according to claim 17 a small amount of the fluid to be tested and examining said coating for visual evidence of the presence of said urobilinogen bodies.
Claim 18
Dependent Claim
17 words
Diagnostic agent according to claim 1 wherein said carrier is a liquid. open-chajned, alicyclic or heterocyclic dialkt54
Claim 19
Dependent Claim
65 words
A method for carrying out an analytical test for the presence of urobilinogen bodies in biological fluids which comprises admixing a diagnostic agent according to claim 19 with a small amount of an ether extract of the fluid to be tested, adding a semisaturated solution of sodium acetate and water and examining the resultant aqueous phase for visual evidence of the presence Of urobilinogen bodies.
Claim 20
Dependent Claim
45 words
Diagnostic agent according to claim 1 wherein said carrier is a paper sheet having a wettable surface impregnated with a solution containing 0.001 to 1 percent of a compound (I) as defined in claim 1 and at least 3 percent of a strong solid acid.
Claim 21
Dependent Claim
31 words
Diagnostic agent according to claim 21 additionally containing at least one member selected from the group consisting of polyvinyl alcohol, polyvinyl pyrrolidone, copolymers of polyvinyl pyrrolidone and polyvinyl acetate and polyglycols.
References Cited (Backward Citations)
US3378346A
Cited
US3446599A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-12-27 | 1975-06-28 | 1975-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-12-27 | 1979-06-28 | 1979-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-12-27 | 1983-06-28 | 1983-12-28 | $7700.00 | Window Closed / Lapsed |