US3627640A
Expired / Lapsed
US
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Enzyme purification and decolorization
Assignee: Yeda Research and Development Co Ltd
•
Filing: 1968-09-27
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Granted: 1971-12-14
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Expires: 1988-09-27
Abstract
Abstract Method of purifying and decoloring a relatively crude enzyme material, e.g., enzyme or enzyme derivative, by forming a polymer-enzyme product.
Inventors
- 👤 Shmaryahu Blumberg
- 👤 Ephraim Katchalski
- 👤 Leon Goldstein
- 👤 Yehuda Levin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 07:21
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
12 words
The process of claim 1, wherein the polymer is an EMA-type polymer.
Claim 2
Dependent Claim
14 words
The process of claim 1, which comprises the step of aqueously washing the product.
Claim 3
Dependent Claim
28 words
The process of claim 1, wherein the enzyme material comprises an enzyme having a sulfhydryl group or a metal derivative thereof and the polymer is an EMA-type polymer.
Claim 4
Dependent Claim
29 words
The process of claim 1, wherein the starting enzyme material and the polymeric enzyme product are both at least partially inactivated and the polymeric enzyme product is subsequently reactivated.
Claim 5
Dependent Claim
21 words
The process of claim 1, wherein the enzyme material comprises a bivalent metal derivative of papain and the polymer is EMA.
Claim 6
Dependent Claim
16 words
The process of claim 1, wherein the enzyme material comprises papain and the polymer is EMA.
Claim 7
Dependent Claim
19 words
The process of claim 1, wherein the enzyme material comprises mercuripapain or zinc papain and the polymer is EMA.
Claim 8
Dependent Claim
31 words
The process of claim 1, wherein the enzyme is selected from the group consisting of papain, chymopapain, ficin, bromelain, asclepain, cellulase, lipase, protease, and enzyme mixtures produced by Bacillus subtilis micro-organisms.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-12-13 | 1975-06-14 | 1975-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-12-13 | 1979-06-14 | 1979-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-12-13 | 1983-06-14 | 1983-12-14 | $7700.00 | Window Closed / Lapsed |