US3607857A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process of removing acrinol from gamma globulin using siliceous material such as silica gel
Assignee: Pharmacia and Upjohn Co
•
Filing: 1970-03-03
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Granted: 1971-09-21
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Expires: 1990-03-03
Abstract
Abstract Removal of acrinol (ethacridine lactate monohydrate) from blood proteins, especially Y-globulin, is accomplished by treating aqueous acrinol solutions of the proteins with a siliceous material which preferentially absorbs the acrinol. Free of acrinol, Y-globulin from sera or plasma especially immune sera or plasma is useful in combatting rejection of tissue and organ transplants and in replacement management of gamma globulin.
Inventors
- 👤 John W Nelson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 00:52
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; C07K16/065; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/837
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
25 words
mixing with said soluble portion at a pH of from about 7 to about 8 human erythrocyte stroma or erythrocytes and recovering the soluble portion;
Claim 2
Dependent Claim
22 words
The process of claim 1 wherein the serum or plasma is that of an animal treated with lymphoid tissue of human origin.
Claim 3
Dependent Claim
18 words
The process of claim 1 wherein the serum or plasma is from a horse treated with human thymocytes.
Claim 4
Dependent Claim
51 words
precipitation gamma globulin from the soluble portion at a pH of from about 7 to about 8 by adding thereto a sufficient quantity of acetone or a lower alkanol to provide an acetone or lower alkanol concentration of from about 20 percent to about 30 percent v./v. and recovering the precipitate;
Claim 5
Dependent Claim
31 words
dissolving the precipitated gamma globulin in phosphate buffer at a pH of from about 6.5 to about 7.5 and treating the resulting solution with silica gel and recovering the soluble portion;
Claim 6
Dependent Claim
11 words
The process of claim 1 wherein the adsorbent is silica gel.
Claim 7
Dependent Claim
14 words
The process of claim 1 wherein the serum or plasma is of human origin.
Claim 8
Dependent Claim
28 words
passing the soluble portion over diethylaminoethyl cellulose equilibrated with phosphate buffer at about pH 6.7 and eluting the diethylaminoethyl cellulose with phosphate buffer at about pH 6.7; and
Claim 9
Dependent Claim
16 words
recovering gamma globulin from the eluate in accordance with measurements of adsorbancies at 280 Mu .
Claim 10
Dependent Claim
13 words
A process of purifying gamma -globulin from serum or plasma consisting essentially of:
Claim 11
Dependent Claim
22 words
The process of claim 6 wherein the serum or plasma is that of an animal treated with lymphoid tissue of human origin.
Claim 12
Dependent Claim
18 words
The process of claim 6 wherein the serum or plasma is from a horse treated with human thymocytes.
Claim 13
Dependent Claim
14 words
The process of claim 6 wherein the serum or plasma is of human origin.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-09-20 | 1975-03-22 | 1975-09-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-09-20 | 1979-03-22 | 1979-09-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-09-20 | 1983-03-22 | 1983-09-21 | $7700.00 | Window Closed / Lapsed |