US3604927A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Total reflection fluorescence spectroscopy
Assignee: Bio Rad Laboratories Inc
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Filing: 1966-11-16
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Granted: 1971-09-14
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Expires: 1986-11-16
Abstract
Abstract A fluorescence spectroscopic device and method of using an attenuated, total, multiple reflection slab on one surface of which a sample is placed. A beam of fluorescence-exciting radiation is passed through the slab by multiple reflections. A detector is located alongside the slab and out of the path of any of the exciting radiation to pick up fluorescent emission passing through the cell and across the path of the exciting radiation. The slab thereby serves as a no-loss secondary filter with respect to the fluorescence, permitting the latter to be easily distinguishable from the exciting radiation even where the wavelengths are similar.
Inventors
- 👤 Tomas Hirschfeld
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 06:41
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/645; G01N21/648; G01N21/17; G01N21/55; G01N21/552
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
28 words
Method as defined in claim 1 including the steps of maintaining said beam at a fixed wavelength band; and measuring said fluorescent radiation across a range of wavelengths.
Claim 2
Dependent Claim
32 words
Methods as defined in claim 1 including the steps of changing the wavelength band of said beam through a range of wavelengths and measuring said fluorescent radiation within a fixed wavelength band.
Claim 3
Dependent Claim
42 words
Method as defined in claim 1 wherein said interface is formed by establishing on said one reflecting surface a coating of material which can extract said sample from a dilute solution of the latter, and applying said dilute solution to said coating.
Claim 4
Dependent Claim
21 words
Method as defined in claim 1 including the step of varying the angle of incidence of said beam on said interface.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-09-13 | 1975-03-15 | 1975-09-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-09-13 | 1979-03-15 | 1979-09-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-09-13 | 1983-03-15 | 1983-09-14 | $7700.00 | Window Closed / Lapsed |