Purification and fractionation of protease and amylase activities in enzyme mixture

Abstract

Abstract ADDITION OF SOLUBLE CALCIUM SALT, SUCH AS CALCIUM ACETATE, AT MODERATELY HIGH LEVELS, E.G., ABOUT 1-2% WEIGHT/ VOLUME, TO AN ENZYME SOLUTION, E.G., A CLARIFIED FERMENTATION BEER, OR AQUEOUS SOLUTION OF REDISOLVED SOLIDS PRECIPITATED FROM SUCH BEER, CONTAINING PROTEASE OR AMYLASE TOGETHER WITH PROTEINACEOUS IMPURITIES, PRECIPITATES PROTEINACEOUS IMPURITIES DIRECTLY WITHOUT REMOVING ENZYMES FROM SOLUTION. ADDITION OF SOLVENT PRIOR TO, CONCURRENTLY WITH, OR AFTER CALCIUM SALT PRECIPITATION, PRECIPITATES AMVLASE FROM SOLUTION. ADDITION OF FURTHER SOLVENT PRECIPITATES PROTEASE FROM SOLUTION. ALLOWS CONVENIENT REMOVAL OF PROTEINACEOUS IMPURITIES, ISOLATION OF AMYLASE IN AN AMYLASE-RICH FRACTION WITH OR WITHOUT THE CALCIUM SALT PRECIPITATED IMPURITIES, AS DESIRED, AND ISOLATION OF PROTEASE AND, THUS PERMITS BOTH PURIFICATION AND FRACTIONATION OF THE ENZYMATIC ACTIVITY IN A CONVENIENT MANNER.

Inventors

• 👤 Leonard Keay
• 👤 Richard G Anderson

Bibliographic Details