Patent US5263957A successfully refreshed from Google Patents.
US3579999A
Expired / Lapsed
US
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Method and apparatus for the preservation of biological substances
Assignee: Redeco Inc
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Filing: 1968-08-08
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Granted: 1971-05-25
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Expires: 1988-08-08
Abstract
Abstract Biological substances such as blood are stored under pressure at below 0* C. temperatures, in a substantially nondilatable vessel. Upon being introduced into the vessel, the temperature of the vessel and the blood are reduced to 4* C.; when equilibrium has been achieved the blood within the vessel is precompressed at a controlled rate several thousand pounds per square inch by the application of mechanical leverage to a vessel piston. Thereafter, the vessel and blood are reduced to a final temperature from 0* C. to -21* C. This temperature reduction, because of the conjunctive effect of the volumetric expansion of the blood and nondilation of the vessel, self-generates a pressure from 15 to 35,000 p.s.i. The vessel may now be stored at the final temperature and the blood within it maintained without deleterious effects.
Inventors
- 👤 Ralph E Schwartz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:43
CPC Classifications
A; A61; A61J; A61J1/00; A61J1/05; A61J1/10; A01; A01N; A01N1/00; A01N1/10; A01N1/16; A01N1/165; A61M; A61M1/00; A61M1/02; A61M1/0272
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
37 words
The method of storing blood claimed in claim 1, comprising the further steps of precompressing said blood in stages subsequent to said initial precompression step at decreasing predetermined temperatures, the last predetermined temperature being the storage temperature.
Claim 2
Dependent Claim
17 words
The method of storing blood claimed in claim 1, wherein said predetermined temperature is the storage temperature.
Claim 3
Dependent Claim
30 words
The method of storing blood claimed in claim 1, wherein the precompression pressure ranges up to 12,000 p.s.i., and wherein said final storage temperature is between 0* and -21* C.
Claim 4
Dependent Claim
33 words
The method of storing blood as claimed in claim 1, wherein the pressure and storing temperature are determined in accordance with the curve of FIG. 1 and the area to the left thereof.
Claim 5
Independent Claim
122 words
A method of storing blood comprising the steps of: introducing said blood into a sterile vessel, said vessel having the ability to withstand a selected internal pressure of up to 35,000 p.s.i., and temperature variations from 4* to -21* C. with substantially no change in volume; precompressing the blood in said vessel to a pressure effectively above atmospheric and not exceeding 12,000 p.s.i. at a rate sufficiently low to avoid damaging pressure transients and at a temperature above the freezing point thereof reducing the temperature of said vessel and the blood therein to a storage temperature of between 0* and -21* C. selected to minimize red cell hemolysis at the storage pressure; and storing said blood at said storage temperature and pressure.
Claim 6
Dependent Claim
40 words
The method claimed in claim 6, wherein said precompression pressure is between 4,000 and 6,000 p.s.i., said pressure is applied in one step at a temperature above 0* C., and wherein said sTorage temperature is between -6* and -14* C.
Claim 7
Independent Claim
120 words
A method of storing whole blood comprising the steps of: precompressing said blood at a temperature above the freezing point thereof to a pressure between 15 and 12,000 p.s.i. dependent upon a predetermined lower temperature to be attained during the method, said pressure being effectively above atmospheric and not exceeding 12,000 p.s.i. and being applied at a rate sufficiently low to avoid damaging pressure transients; reducing the temperature of said blood to the storage temperature while maintaining the volume substantially constant, said storage temperature being above that which would permit freezing of said blood at the storage pressure and at or below normal freezing of said blood at atmospheric pressure; and storing said blood at said storage temperature and pressure.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-05-24 | 1974-11-23 | 1975-05-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-05-24 | 1978-11-23 | 1979-05-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-05-24 | 1982-11-23 | 1983-05-25 | $7700.00 | Window Closed / Lapsed |