Method for the detection of virus
Abstract
Abstract THE PRESENCE OR ABSENCE OF VIRUS MAY BE DETECTED BY INOCULATING A TISSUE CULTURE, WHICH IS AN APPROPRIATE HOST FOR THE SUSPECTED VIRUS, WITH A SAMPLE OF THE MATERIAL SUSPECTED TO CONTAIN A VIRUS AND THEN COMPARING THE AMOUNT OF ADENOSINE TRIPHOSPHATE IN THE CULTURE BEFORE AND AFTER A PERIOD OF INCUBATION. THE ADENOSINE TRIPHOSPHATE CONTENT OF THE INOCULATED TISSUE CULTURE IS DETERMINED BY REACTING THE TISSUE CULTURE WITH A MIXTURE COMPRISING LUCIFERIN, LUCIFERASE, AND A CATION IN THE PRESENCE OF OXYGEN AND MONITORING THE AMOUNT OF BIOLUMINESCENCE GIVEN OFF THEREBY. A SIGNIFICANT CHANGE IN THE AMOUNT OF BIOLUMINESCENE AFTER INCUBATION INDICATES THE PRESENCE OF VIRUS, WHEREAS THE ABSENCE OF VIRUS IS INDICATED BY NO SIGNIFICANT CHANGE.
Inventors
- 👤 Emmett W Chappelle
Bibliographic Details
Claims Analysis
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