US3572890A — Microscope slide and spinner

Claim 1

Independent Claim 191 words

Apparatus for examining biological cells comprising: a. a vacuum spinner having a shaft forming a passage therethrough for drawing a vacuum through said passage while rotating said shaft at high speed; b. means forming a support for rotation with said shaft having a platform forming a sleeve around an annulus for pulling a vacuum through said passage and platform while said support is being rotated by said shaft, and a flat, rectangular, plate-shaped slide having a first, major, flat surface for rotation by said support by pulling said vacuum against said surface while said surface is in contact with said platform, and a second, major, flat surface parallel with said first surface forming two, spaced-apart, uniform cross section, open ended grooves, extending continuously and uninterruptedly across said second, major, flat surface whereby a suspension containing biological cells can be placed on said second, major, flat surface between said grooves for rotation of said slide by said vacuum spinner and support for communicating said suspension with said grooves for separating a uniform, substantially undamaged monolayer of said cells from said suspension and depositing said separated cells on said slide between said grooves.

Claim 2

Dependent Claim 191 words

Apparatus for examining biological cells comprising: a. a vacuum spinner having a shaft forming a passage therethrough for drawing a vacuum through said passage while rotating said shaft at high speed; b. means forming a support for rotation with said shaft having a platform forming a sleeve around an annulus for pulling a vacuum through said passage and platform while said support is being rotated by said shaft, and a flat, rectangular, plate-shaped slide having a first, major, flat surface for rotation by said support by pulling said vacuum against said surface while said surface is in contact with said platform, and a second, major, flat surface parallel with said first surface forming two, spaced-apart, uniform cross section, open ended grooves, extending continuously and uninterruptedly across said second, major, flat surface whereby a suspension containing biological cells can be placed on said second, major, flat surface between said grooves for rotation of said slide by said vacuum spinner and support for communicating said suspension with said grooves for separating a uniform, substantially undamaged monolayer of said cells from said suspension and depositing said separated cells on said slide between said grooves.

Claim 3

Dependent Claim 96 words

The invention of claim 1 in which said platform forms below a shoulder a depressed area whose depth is less than the thickness of said slide, which corresponds to the distance between said first and second, major, flat surfaces, whereby cells containing radioactive material can be applied to said slide and an excess thereof can be removed from said slide by centrifugal force during the rotation of said slide by carrying said excess over said shoulder so as to prevent the accumulation of radioactivity containing material on said suppoRt, said shoulder and in said depressed area.

Claim 4

Dependent Claim 119 words

The invention of claim 1 in which said slide is a 1-inch X 3-inch X 0.040-inch glass, rectangular, plate-shaped, microscope slide, said grooves are two 1-inch long, grooves 0.010-inch wide by 0.010-inch deep below the surface of said first, major flat surface and uniformly 1-inch apart for receiving said cell-containing suspension in an inch square area between said grooves, whereby a flow of said cell-containing suspension along said first, major, flat surface is produced by the rotation of said slide and is confined for transportation by said grooves for the uniform separation distribution and deposit of said monolayer of said cells on said slide between said grooves during said rotation without substantial deflection of said slide by said vacuum.

Claim 5

Dependent Claim 114 words

The apparatus of claim 1 for the preparation of biological cells of the type deposited from sera having different viscosities and cell sizes used in microscopic and autoradiographic blood-cell studies, wherein said grooves are formed intaglio at equal angles to long edges on said first, major, flat, side to provide an inch square flat area the center of which is supported by said support coaxial with the axis of rotation of said shaft for receiving and rotating said slide and cell-containing suspension applied to said slide between said grooves at right angles to said axis to substantial said monolayer of cells on said slide form microscopic as well as automatic examination of said cells.

Claim 6

Dependent Claim 44 words

The apparatus of claim 1 in which said support has raised shoulder means around the perimeter of said slide for holding said slide against lateral movement during said rotation, and for centering said slide symmetrically relative to the axis of rotation of said shaft.

Claim 7

Dependent Claim 53 words

The apparatus of claim 1 in which said support has O-ring means in said sleeve for providing vacuum sealing contact with said shaft for providing for the selective insertion of said shaft into said sleeve for attaining a slide rotation speed of 5000 r.p.m quickly without relative slippage between said shaft and sleeve.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1974-03-29	1974-09-28	1975-03-30	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1978-03-29	1978-09-28	1979-03-30	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1982-03-29	1982-09-28	1983-03-30	$7700.00	Window Closed / Lapsed

Microscope slide and spinner

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