Diagnostic agents for use in the determination of hydroperoxides and of peroxidate-active substances and methods for manufacturing and using the same

Abstract

Abstract THERE ARE DISCLOSED DIAGNOSTIC AGENTS SUITABLE FOR USE IN CARRYING OUT RAPID ANALYTICAL DETERMINATIONS OF THE PRESENCE AND/OR CONCENTRATION OF HYDROPEROXIDE, SUBSTANCES WHICH REACT WITH THE LIBERATION OF JYDROGEN PEROXIDE, PEROXIDASE AND PEROXIDATE-ACTIVE SUBSTANCES. THE DIAGNOSTIC AGENTS OF THE INVENTION ARE ADAPTED FOR USE IN TEST PROCEDURES INVOLVING OPTICAL INSTUMENTS SUCHAS COLORMETERS AND PHOTOMETERS AND ALSO IN TEST PROCEDURES INVOLVING TEST PAPERS. THE DIAGNOSTIC AGENTS AS TAUGHT HEREIN COMPRISE AN INDICATOR I.E., CHROMOGEN, WHICH IS OXIDIZED BY HYDROGEN PEROXIDE AND PEROXIDASE OR PEROSIDATE ACTIVE SUBSTANCES TO FORM A DYESTUFF WHOSE INTENSITY IS DEPENDENT ON THE CONCENTRATION OF THE PEROXIDE, PEROXIDASE OR PEROXIDATE ACTIVE SUBSTANCE PRESENT IN THE TEST SAMPLE. THE INDICATOR I.E., CHROMOGEN IS A COMPOUND OF THE FORMULA: (R4,R5,R6-PHENYL)-C(-R3)=N-(N(-R2))N-C(=X)-R1 WHERIN R1 IS AMINO OR AN ALIPHATIC, ARALIPHATIC, AROMATIC OR HETEROCYCLIC RADICAL WHICH MAY CARRY ONE OR MORE SUBSTITUENTS, OR A GROUP HAVING THE FORMULA: (R4,R5,R6-PHENYL)-C(-R3)=N-(N(-R2))N-C(=X)-(CH(-OH))M- WHEREIN R2 IS HYDROGEN, AN ALIPHATIC, AROMATIC OR HETEROCYCLIC RADICAL WHICH MAY CARRY ONE OR MORE SUBSTITUENTS, R3 IS HYDROGEN, ALKYL OR PHENYL AND MAY BE MONO- OR POLY SUBSTITUTED, R4 IS AMINO, HYDROXYL OR ALKOXY, R5 AND R6 ARE EACH HYDROGEN, AMINO, HYDROXY,ALKOXY OR ALKYL, X IS OXYGEN, SULFUR OR IMINO WHICH MAY BE SUBSTITUTED BY A GROUP AS DEFINED BY R1, N IS 0 OR 1, M IS 0,1 OR 2; AND WHEREIN X AND R1 AND R2 OR R1 AND R3 CAN BE JOINED TOGETHER TO FORM A RING. THE DIAGNOSTIC AGENTS CAN BE USED FOR ANALYTICAL DETERMINATIONS INVOLVING, FOR EXAMPLE, GLUCOSE, GALACTOSE, AMINO ACIDS, URIC ACID, PEROXIDE, HEMOGLOBIN, PEROXIDASE, ETC. IN SAMPLES, FOR EXAMPLE, CONSTITUTING BIOLOGICAL FLUIDS SUCH AS BLOOD, URINE, SPINAL FLUID, ETC., MILK, COSMETIC AND DRUG FORMULATIONS, ETC.

Inventors

• 👤 Hans-Georg Rey
• 👤 Hans Wielinger
• 👤 Peter Rieckmann

Bibliographic Details