Packaging and storing regenerated collagen sutures

Abstract

Abstract REGENERATED COLLAGEN SUTURES OF UNUSUAL STRENGTH AND SURGICALLY DESIRABLE CHARACTERISTICS ARE OBTAINED BY CLEANING YOUNG BEEF TENDONS, GRINDING WHILE FROZEN, TREATING WITH TRYPSIN, WASHING IN WATER, TREATING WITH THE SODIUM SALT OF ETHYLENEDIAMINE TETRACETIC ACID, WASHING WITH WATER, DISPERSING THE COLLAGEN TO FORM AN AQUEOUS GEL, WHICH MAY CONTAIN SOME ETHANOL, WITH HYDROCHLORIC ACID, AT A PH OF ABOUT 2.3 TO 3.3 TO FORM A SPINNABLE GEL OF ABOUT 0.86% SOLIDS, EXTRUDING INTO A DEHYDRATING, COAGULATING AMMONIACAL SOLVENT-WATER BATH, PRESSURE ROLLING TO EXPEL THE SOLVENT-WATER, AND AT LEAST PARTIALLY DRYING. THE SOLVENT MAY BE ETHANOL OR ACETONE. THE RIBBON IS TREATED WITH PYROGALLOL, OR OTHER POLYHYDRIC PHENOL, THE PYROGALLOL TREATED WITH HEXAVALENT CHROMIUM, THEN THE CHROMIUM IS REDUCED FURTHER WITH A BISULFITE, TO THE TRIVALENT STATE, AND THE DOUBLE TANNED RIBBON TWISTED AND STRETCHED AND DRIED TO FORM THE SUTURE.UNUSUAL STRENGTH DEVELOPED IS PROTECTED BY STORING IN A 3-4% WATER IN ISOPROPANOL TUBING FLUID UNTIL READY FOR USE. THE DOUBLE TAN GIVES NOT ONLY A HIGH INITIAL STRENGTH, BUT CAUSES THE SUTURE, WHEN USED, TO RETAIN AN ADEQUATE PROPORTION OF ITS INITIAL STRENGTH FOR AT LEAST 15 DAYS IN MUSCULAR TISSUE. THE SMALLER SIZES HOLD UNTIL HEALING OCCURS IN CORNEAL TISSUE IN EYE OPERATIONS.

Inventors

• 👤 Richard Joseph Duffy
• 👤 Francesco Demaria

Bibliographic Details