Electrophoretic separation utilizing liquid centrifuge
Abstract
Abstract A METHOD AND APPARATUS ARE DESCRIBED FOR SEPARATING MACROMOLECULAR COMPONENTS BY ELECTROPHORESIS IN A LIQUID DENSITY GRADIENT WHILE IT IS BEING STABILIZED IN A CENTRIFUGAL FIELD. A MODIFIED PANCAKE-TYPE CENTRIFUGE ROTOR IS USED WHICH CONTAINS TWO RADIALLY EXTENDING COMPARTMENTS IN OPEN COMMUNICATION AT THEIR RADICAL PERIPHERIES. ELECTRODES, IN ELECTRICAL COMMUNICATION WITH EXTERNALLY MOUNTED CONTACT OR COMMUTATOR RINGS, ARE DISPOSED WITHIN THE AXIAL REGION OF EACH COMPARTMENT. WHILE THE MOTOR IS ROTATING, A LIQUID DENSITY GRADIENT AND THEN A SAMPLE ARE INTRODUCED INTO AT LEAST ONE ROTOR COMPARTMENT AND DIRECT CURRENT APPLIED TO THE ELECTRODES CAUSING ELECTROPHORETIC SEPARATION OF SEPARABLE COMPONENTS CONTAINED IN THE SAMPLE. THE LIQUID DENSITY GRADIENT AND SEPARATED COMPONENTS CONTAINED THEREIN ARE THEN REMOVED FROM THE ROTOR BY CONVENTIONAL DISPLACEMENT TECHNIQUES.
Inventors
- 👤 Norman G Anderson
Bibliographic Details
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