Process for producing rubella-virus hemagglutinating antigen
Abstract
Abstract A PROCESS IS PROVIDED FOR PRODUCING HIGH TITER RUBELLAVIRUS HEMAGGLUTINATING ANTIGEN. RUBELLA-VIRUS HEMAGGLUTININ IS FIRST PREPARED BY INCOULATING A TISSUE CULTURE OF BABY HAMSTER KIDNEY CELLS MAINTAINED IN A SERUM-FREE, NUTRIENT TISSUE CULTURE FLUID WITH INFECTIOUS RUBELLA VIRUS AND INCUBATING THE INCOULATED CELLS TO PROPAGATE THE VIRUS IN THE CELLS. AFTER INCUBATION, THE PH OF THE FLUID IS ADJUSTED TO BETWEEN ABOUT 9 AND 10 TO PRODUCE HIGH TITER RUBELLA-VIRUS HEMAGGLUTININ. THE ANTIGEN IS THEN SPRAYED BY DESTROYING THE INFECTIVITY OF THE VIRUS WITHOUT DESTROYING ITS ANTIGENICITY. THE ANTIGEN IS USEFUL IN THE DETECTION OF RUBELLA IMMUNITY AND RUBELLA INFECTION AND IN THE EVALUATION OF POTENTIAL RUBELLA VACCINES.
Inventors
- 👤 Sergio N Delgado
Bibliographic Details
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