US3413208A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of streptokinase by zone electrophoresis using a density-gradient column
Assignee: Wyeth Holdings LLC
•
Filing: 1963-02-19
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Granted: 1968-11-26
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Expires: 1983-02-19
Abstract
No abstract record available.
Inventors
- 👤 Jr William Frederic Barg
- 👤 Bell Paul Hadley
- 👤 Davies Maurice Charles
- 👤 Renzo Edward Clarence De
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 17:07
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/4476; C; C07; C07K; C07K14/00; C07K14/195; C07K14/315; C07K14/3153
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
98 words
A PROCESS OF PURIFICATION OF STREPTOKINASE COMPRISING IN COMBINATION, (A) ESTABLISHING AN ELECTROPHORETIC CHANNEL, (B) MAINTAINING IN THE CHANNEL A VARIABLE DENSITYGRADIENT SOLUTION IN BUFFER FROM TOP TO BOTTOM, THE SOLUTION BEING OF INERT NON-ELECTROLYTE, PRODUCING A DIRECT CURRENT FLOW FROM THE CATHODE IN THE TOP TO AN ANODE IN THE BOTTOM OF THE CHANNEL, (C) INTRODUCING A SOLUTION OF STREPTOKINASE COMPOSITION, DISSOLVED IN A BUFFER, NEAR THE CATHODE, THE BUFFER BUFFERING TO A PH OF FROM ABOUT 7 TO 8, (D) CONTINUING THE FLOW OF CURRENT TO PRODUCE ELECTROPHORESIS UNTIL SUBSTANTIAL SEPARATION OF PROTEIN CONSTITUENTS RESULTS, AND
Claim 2
Dependent Claim
98 words
A PROCESS OF PURIFICATION OF STREPTOKINASE COMPRISING IN COMBINATION, (A) ESTABLISHING AN ELECTROPHORETIC CHANNEL, (B) MAINTAINING IN THE CHANNEL A VARIABLE DENSITYGRADIENT SOLUTION IN BUFFER FROM TOP TO BOTTOM, THE SOLUTION BEING OF INERT NON-ELECTROLYTE, PRODUCING A DIRECT CURRENT FLOW FROM THE CATHODE IN THE TOP TO AN ANODE IN THE BOTTOM OF THE CHANNEL, (C) INTRODUCING A SOLUTION OF STREPTOKINASE COMPOSITION, DISSOLVED IN A BUFFER, NEAR THE CATHODE, THE BUFFER BUFFERING TO A PH OF FROM ABOUT 7 TO 8, (D) CONTINUING THE FLOW OF CURRENT TO PRODUCE ELECTROPHORESIS UNTIL SUBSTANTIAL SEPARATION OF PROTEIN CONSTITUENTS RESULTS, AND
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1971-11-26 | 1972-05-27 | 1972-11-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1975-11-26 | 1976-05-27 | 1976-11-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1979-11-26 | 1980-05-27 | 1980-11-26 | $7700.00 | Window Closed / Lapsed |