US3410756A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Naphthyl esters as fluorogenic substrates for enzymes
Assignee: United States Department of the Army
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Filing: 1966-05-27
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Granted: 1968-11-12
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Expires: 1986-05-27
Abstract
No abstract record available.
Inventors
- 👤 George G Guilbault
- 👤 David N Kramer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 05:43
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/44; C12Q1/46; C12Q1/42; C12Q2334/00; C12Q2334/30
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
106 words
A PROCESS OF MEASURING ENZYME ACTIVITY AT A LOW RANGE OF SUBSTRATE CONCENTRATIONS WHICH CONSISTS OF THE STEPS OF: (A) HYDROLYZING IN THE PRESENCE OF MACLLVAINE BUFFER, PH 7-40, A FLUOROGENIC SUBSTRATE TAKEN FROM THE GROUP CONSISTING OF ACETYL AND BUTYRYL ESTERS OF A AND B-NAPHTHOL AND B-NAPHTHYL PHOSPHATE WITH AN ENZYME TAKEN FROM THE GROUP CONSISTING OF CHOLINESTERASE, ACYLASE, AND ACID PHOSPHATASE TO PRODUCE HIGHLY FLUORESCENT MATERIAL; (B) REGISTERING THE RATE OF CHANGE IN FLUORESCENCE WITH TIME, $F/$T; (C) DETERMINING THE SLOPE OF THE CURVE $F/$T; (D) RECORDING THE CALIBRATION PLOTS OF $F/$T VERSUS ENZYME CONCENTRATION WHEREBY THE UNKNOWN CONCENTRATION OF ENZYME MAY BE DETERMINED.
Claim 2
Dependent Claim
106 words
A PROCESS OF MEASURING ENZYME ACTIVITY AT A LOW RANGE OF SUBSTRATE CONCENTRATIONS WHICH CONSISTS OF THE STEPS OF: (A) HYDROLYZING IN THE PRESENCE OF MACLLVAINE BUFFER, PH 7-40, A FLUOROGENIC SUBSTRATE TAKEN FROM THE GROUP CONSISTING OF ACETYL AND BUTYRYL ESTERS OF A AND B-NAPHTHOL AND B-NAPHTHYL PHOSPHATE WITH AN ENZYME TAKEN FROM THE GROUP CONSISTING OF CHOLINESTERASE, ACYLASE, AND ACID PHOSPHATASE TO PRODUCE HIGHLY FLUORESCENT MATERIAL; (B) REGISTERING THE RATE OF CHANGE IN FLUORESCENCE WITH TIME, $F/$T; (C) DETERMINING THE SLOPE OF THE CURVE $F/$T; (D) RECORDING THE CALIBRATION PLOTS OF $F/$T VERSUS ENZYME CONCENTRATION WHEREBY THE UNKNOWN CONCENTRATION OF ENZYME MAY BE DETERMINED.
References Cited (Backward Citations)
US3049411A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1971-11-12 | 1972-05-13 | 1972-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1975-11-12 | 1976-05-13 | 1976-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1979-11-12 | 1980-05-13 | 1980-11-12 | $7700.00 | Window Closed / Lapsed |