Antibiotic av290 and production thereof

Abstract

Abstract 1,194,365. Antibiotic AV290. AMERICAN CYANAMID CO. 30 June, 1967 [29 July, 1966], No. 30424/67. Heading C2A. The invention relates to an antibacterial antibiotic, antibiotic AV290, its salts and hydrolysis product having the following properties: AV290 base and sulphate are soluble in water and dimethylsulphoxide, moderately soluble in methanol, insoluble in other common organic solvents. Antibiotic AV290 is produced by cultivating Streptomyces candidus NRRL 3218, or an antibiotic AV290 producing mutant thereof, in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic salts under submerged aerobic conditions. The fermentation broth is clarified at pH 6À0, treated with activated carbon, washed and eluted with 40% aqueous acetone of pH 2À0. After concentrating, Ba(OH) 2 is added to pH 5À5, the precipitated BaSO 4 removed and the liquid is treated with acidified alumina of pH 3À0. 50% aqueous methanol is used for elution, followed by addition of Ba(OH) 2 to pH 6À3 to precipitate excess SO 4 ions and crude AV290 is recovered. Further purification is by adsorption on Amberlite 1RC-50 (Trade Mark) or CM Sephadex C-25 (Trade Mark) and elution with dilute H 2 SO 4 or aqueous NaCl. Antibiotic AV290 sulphate is converted to the base by treatment with a mixture of Amberlite 1R45 (OH-) and Amberlite 1R20 (H+). The hydrolysis product is obtained by boiling AV290 base with aqueous HCl. A therapeutic composition for administration to humans and animals comprises antibiotic AV290, a salt thereof, or the acid hydrolysis product and a carrier, e.g. for topical or parenteral use.

Inventors

• 👤 Martin P Kunstmann
• 👤 Porter John Norman

Bibliographic Details