US3331752A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Determination of dehydrogenase
Assignee: Ortho Pharmaceutical Corp
•
Filing: 1966-06-07
•
Granted: 1967-07-18
•
Expires: 1986-06-07
Abstract
No abstract record available.
Inventors
- 👤 Jr Jacob Struck
- 👤 John K Inman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 22:25
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
125 words
A METHOD OF DETERMINING THE CONCENTRATION IN BODY FLUID OF NICOTINAMIDE-ADENINE NUCLEOTIDE DEPENDENT ENZYMES WHICH COMPRISES INCUBATING IN A SMALL VOLUME OF BODY FLUID A BUFFERED REDUCED SUBSTRATE SELECTED FROM THE GROUP CONSISTING OF LACTIC ACID AND LACTIC ACID SALTS, MALIC ACID AND MALIC ACID SALTS, A-HYDROXYBUTYRIC ACID AND AHYDROXYBUTYRIC ACID SALTS, SORBITOL, ISOCITRIC ACID AND ISOCITRIC ACID SALS AND ETHANOL, AND AN OXIDIZED COENZYME SELECTED FROM THE GROUP CONSISTING OF NICOTINAMIDE-ADENINE INUCLEOTIDE-ADENINE DINUCLEOTIDE PHOSPHATE AND A DIVALENT CATION, REDUCING THE PH OF THE REACTION MIXTURE TO A PH BETWEEN ABOUT 2.8 AND ABOUT 5.0 AND ADDING A REDUCIBLE CLOR DEVELOPING METAL CHELATE SELECTED FROM THE GROUP CONSISTING OF FERRI CHELATES OF 1.10-PHENANTHROLINE, 4,7-DIPHENYL1,1.0-PHENANTHROLINE AND SULFONATED 4,7-DIPHENYL-1,10PHENANTHROLINE AND CUPRIC CHELATES OF 2.9-DIMETHYL-1,10PHENANTHROLINE AND SULFONATED 2,9-DIMETHYL-4,7-DIPHENYL-1,10-PHENANTHROLINE.
Claim 2
Independent Claim
125 words
A METHOD OF DETERMINING THE CONCENTRATION IN BODY FLUID OF NICOTINAMIDE-ADENINE NUCLEOTIDE DEPENDENT ENZYMES WHICH COMPRISES INCUBATING IN A SMALL VOLUME OF BODY FLUID A BUFFERED REDUCED SUBSTRATE SELECTED FROM THE GROUP CONSISTING OF LACTIC ACID AND LACTIC ACID SALTS, MALIC ACID AND MALIC ACID SALTS, A-HYDROXYBUTYRIC ACID AND AHYDROXYBUTYRIC ACID SALTS, SORBITOL, ISOCITRIC ACID AND ISOCITRIC ACID SALS AND ETHANOL, AND AN OXIDIZED COENZYME SELECTED FROM THE GROUP CONSISTING OF NICOTINAMIDE-ADENINE INUCLEOTIDE-ADENINE DINUCLEOTIDE PHOSPHATE AND A DIVALENT CATION, REDUCING THE PH OF THE REACTION MIXTURE TO A PH BETWEEN ABOUT 2.8 AND ABOUT 5.0 AND ADDING A REDUCIBLE CLOR DEVELOPING METAL CHELATE SELECTED FROM THE GROUP CONSISTING OF FERRI CHELATES OF 1.10-PHENANTHROLINE, 4,7-DIPHENYL1,1.0-PHENANTHROLINE AND SULFONATED 4,7-DIPHENYL-1,10PHENANTHROLINE AND CUPRIC CHELATES OF 2.9-DIMETHYL-1,10PHENANTHROLINE AND SULFONATED 2,9-DIMETHYL-4,7-DIPHENYL-1,10-PHENANTHROLINE.
References Cited (Backward Citations)
US2996436A
Cited
US2999052A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1970-07-17 | 1971-01-16 | 1971-07-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1974-07-17 | 1975-01-16 | 1975-07-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1978-07-17 | 1979-01-16 | 1979-07-18 | $7700.00 | Window Closed / Lapsed |