Pituitary extract

Claim 1

A PROCESS OF OBTAINING ADRENOCORTICOTROPIN WHICH COMPRISES: (1) EXTRACTING MAMMALIAN PITUITARY GLANDS WITH ACIDIFIED AQUEOUS ACETONE AND RECOVERING THE EXTRACT; (2) MIXING THE RECOVERED EXTRACT WITH EXCESS ACETONE TO PRECIPITATE AN INSOLUBLE PRODUCT; (3) PREPARING AN AQUEOUS SOLUTION OF THE INSOLUBLE PRODUCT AT ABOUT PH 3 AND ADDING SALT TO SATURATE THE AQUEOUS SOLUTION AND FORM A PRECIPITATE; (4) PREPARING AN AQUEOUS SOLUTION OF THE PRECIPITATE AND DIALYZING THE SOLUTION AGAINST WATER; (5) RECOVERING A PRELIMINARY ADRENOCORTICOTROIC PRODUCT FROM THE DIALYZED SOLUTION BY VLATILIZING WATER THEREFROM IN VACUO; (6) DISSOLVING THE RECOVERED PRODUCT IN AN AQUEOUS SOLUTION OF AMMONIUM ACETATE OF 0.01 M AND PH 4.6 AND CHROMATOGRAPHING THE SOLUTION ON CARBOXYMETHYLCELLULOSE; (7) FRACTINALY ELUTING ABSORBED MATERIAL FROM THE CARBOXYMETHYLCELLULOSE WITH SEQUENTIAL CONTINUOUS LINEAR GRADIENTS TO AMMONIUM ACETATE OF 0.1 M, PH 6.7 AND 0.2 M, PH 6.7; AND (8) RECOVERING ADRENOCORTICOTROPIN FROM COMBINED TERMINAL FRACTINAL ELUATES IN TEH CONTINUOUS LINEAR GRADIENT TO AMMONIUM ACETATE OF 0.2 M AND PH 6.7 BY VOLATILIZING WATER ND AMMONIUM ACETATE THEREFROM IN VACUO.