Physiologically active proteinic substance and method of preparing same from animal salivary glands

Claim 1

THE PHYSIOLOGICALLY ACTIVE SUBSTANCE ACTIVE IN DECREASING THE CALCIUM LEVEL IN BLOOD SERUM AND FOR INCREASING THE LEUKOCYTE NUMBER IN CIRCULATING BLOOD, WHICH HAS A MOLECULAR WEIGHT OF 62,000, AN ISOELECTRIC POINT DEFINED AS PH=3.0-3.5, AN ELEMENTARY ANALYSIS: C= 45.04%; H=6.77%; N=13.15%; AND S=0.71%, A CONSTITUENT AMINO ACID GROUP CONSISTING OF GLYCINE, ALANINE, VALINE, LEUCINE, PHENYL ALANINE, ASPARTIC ACID, GLUTAMIC ACID, ARGININE, LYSINE, HISTIDINE, CYSTINE, METHIONINE, SERINE, THERONINE, PROLINE, TRYPTOPHAN AND TYROSINE, A CONSTITUENT SUGAR GROUP CONSISTING OF GALACTOSE AND GALACTOSAMINE; AND WHICH SUBSTANCE HAS THE UV-SPECTRUM SHOWN IN FIGURE 5 OF THE ACCOMPANING DRAWINGS, SHOWING NO MAXIMUM ABSORPTION WITHIN THE RANGE OF THE WAVE LENGTH OF FROM 240 MU TO 300 MU, PREPARED BY A PROCESS WHEREIN ISOLATED SALIVARY GLAND IS EXTRACTED WITH WATER AT PH 8.0, FOLLOWED BY THE STEPS OF ADJUSTING THE HYDROGEN ION CONCENTRATION OF THE EXTRACT TO EFFECT PRECIPITATION AT THE ISOELECTRIC POINT; EXTRACTING THE RESULTANT PRECIPITATE WITH WATER AT PH 8.0; FREEZING AND THEN THAWING THE EXTRACT; ADJUSTING THE HYDROGEN ION CONCENTRATION OF THE RESULTING CLEAR THAWED LIQUOR TO EFFECT PRECIPITATION OF EFFECTIVE COMPONENT AT THE ISOELECTRIC POINT; AND SUBJECTING THE RESULTING PRECIPITATE TO FRACTIONATION TO RECOVER PURE A-PAROTIN.