Method of increasing the viscosity of an aqueous solution of a deacetylated polysaccharide

Claim 1

A METHOD OFF SUBSTANTIALLY INCREASING THE VISCOSITY OF A 0.25 TO 1.0 PERCENT AQUEOUS SOLUTION OF THE ALKALIDEACETYLATED DERIVATIVE OF THE NATIVE POLYSACCHARIDE B-1459 COMPRISING ADDING ABOUT FROM 0.25 TO 5.0 PERCENT OF A MEMBER SELECTED FROM THE GROUP CONSISTING OF BORAX, CALCIUM ACETATE, AND POTASSIUM CHLORIDE THERETO, SAID NATIVE POLYSACCHARIDE B-1459 HAVING BEEN PRECIPITATED BY THE ADDITION OF METHANOL AND POTASSIUM CHLORIDE TO PROVIDE RESPECTIVE CONCENTRATIONS OF 56 PERCENT AND 2 PERCENT THEREOF IN A CENTRIFUGED 72 TO 96 HOUR AEROBIC WHOLE CULTURE FERMENTATION OF XANTHOMONAS COMPESTRIS NRRL B-1459 AND THEN ISOLATED, TTHE ALKALIDEACETYLATED DERIVATIVE OF SAID POLYSACCHARIDE B-1459 HAVING BEEN OBTAINED BY DISSOLVING, UNDER NITROGEN, 0.67 PART OF THE ISOLATED NATIVE POLYSACCHARIDE B-1459 IN 100 PARRTS OF AIR-FREE WATER CONTAINING 1.35 PARTS OF POTASSIUM CHLORIDE, VIGOROUSLY STIRRING IN 34 PARTS OF 0.20 N POTASSIUM HYDROXIDE SOLUTION, MAINTAINING THE MIXTURE AT 25*C. FOR 2 HOURS UNDER NITROGEN, ADJUSTING THE PH TO NEUTRALITY, PRECIPITTATING THE DEACETYLATED POLYSACCHARIDE BY ADDING METHANOL TO PROVIDE A CONCENTRATION THEREOF OF 44 PERCENT BY WEIGHT, AND ISOLATING THE DEACETYLATED POLYSACCHARIDE.

Claim 2

A METHOD OFF SUBSTANTIALLY INCREASING THE VISCOSITY OF A 0.25 TO 1.0 PERCENT AQUEOUS SOLUTION OF THE ALKALIDEACETYLATED DERIVATIVE OF THE NATIVE POLYSACCHARIDE B-1459 COMPRISING ADDING ABOUT FROM 0.25 TO 5.0 PERCENT OF A MEMBER SELECTED FROM THE GROUP CONSISTING OF BORAX, CALCIUM ACETATE, AND POTASSIUM CHLORIDE THERETO, SAID NATIVE POLYSACCHARIDE B-1459 HAVING BEEN PRECIPITATED BY THE ADDITION OF METHANOL AND POTASSIUM CHLORIDE TO PROVIDE RESPECTIVE CONCENTRATIONS OF 56 PERCENT AND 2 PERCENT THEREOF IN A CENTRIFUGED 72 TO 96 HOUR AEROBIC WHOLE CULTURE FERMENTATION OF XANTHOMONAS COMPESTRIS NRRL B-1459 AND THEN ISOLATED, TTHE ALKALIDEACETYLATED DERIVATIVE OF SAID POLYSACCHARIDE B-1459 HAVING BEEN OBTAINED BY DISSOLVING, UNDER NITROGEN, 0.67 PART OF THE ISOLATED NATIVE POLYSACCHARIDE B-1459 IN 100 PARRTS OF AIR-FREE WATER CONTAINING 1.35 PARTS OF POTASSIUM CHLORIDE, VIGOROUSLY STIRRING IN 34 PARTS OF 0.20 N POTASSIUM HYDROXIDE SOLUTION, MAINTAINING THE MIXTURE AT 25*C. FOR 2 HOURS UNDER NITROGEN, ADJUSTING THE PH TO NEUTRALITY, PRECIPITTATING THE DEACETYLATED POLYSACCHARIDE BY ADDING METHANOL TO PROVIDE A CONCENTRATION THEREOF OF 44 PERCENT BY WEIGHT, AND ISOLATING THE DEACETYLATED POLYSACCHARIDE.