Processes in peptide synthesis

Claim 2

A PROCESS FOR THE PRODUCTION OF AN ALPHA-(N-TERTIARYBUTYLOXYCARBBONYL) -NATURALLY OCCURING AMINO ACID COMPRISING THE STEPS OF REACTING A NATURALLY OCCURING AMINO ACID WITH PARA-NITROPHENYL TERTIARY BUTYL CARBONATE IN THE PRESENCE OF A WATER-MISCIBLE ORGANIC SOLVENT SELECTED FROM THE GROUP CONSISTING OF LOWER ALKYL ALCOHOLS AND DI-(LOWER)ALKYL KETONES AT A PH OF BETWEEN 8 TO 12.

Claim 3

A PROCESS FOR PREPARING AN ALPHA-(N-TERTIARY BUTYLOXY CARBONYL) TRIPEPTIDE LOWER ALKYL ESTER COMPRISING THE STEPS OF (A) REACTING A MEMBER OF THE GROUP CONSISTING OF ALPHA-N-TERTIARYBUTYLOXYCARBONYL) -NATURALLY OCCURRIING AMINO ACIDS WITH A MEMBER SELECTED FROM THE GROUP CONSISTING OF LOWER ALKYL ESTER OF ALPHA NATURALLY OCCURING AMINO ACIDS IN THE PRESENCE OF A PEPTIDE REACTION-PROMOTING AGENT CONSISTING OF A LOWER ALKYL PYROPHOSPHITE AND A PHOSPHITE HYDROGEN HALIDE ACCEPTOR SELECTED FROM THE GROUP CONSISTING OF DI-)LOWER ALKYL PHOSPHITES, TRI-(LOWER ALKYL) PHOSPHITES, TRI-(LOWER ALKENYL) PHOSPHITES HAVING ALLYLIC UNSATURATION AND LOWER ALKYL ALKYLENE PHOSPHITES; (B) SUBJECTING THE PRODUCT OF SAID STEP (A), WHILE SAID PRODUCT IS STILL IN THE PEPTIDE-FORMING MEDIUM OF SAID STEP (A), TO THE ACTION OF A HYDROGEN HALIDE SELECTED FROM THE GROUP CONSISTING OF HYDROGEN BROMIDE AND HYDROGEN CHLORIDE WHEREBY THE TERTIARY BUTYLOXYCARBONYL ALPHA N-BLOCKING GROUP IS REMOVED; AND (C) REACTING THE PRODUCT OF SAID STEP (B), WHILE SAID PRODUCT OF SAID STEP (B) IS STILL IN THE REACTION MEDIUM OF SAID STEP (B), WITH A MEMBER OF THE GROUP CONSISTING OF ALPHA-(N-TERTIARY BUTYLOXY CARBONYL)NATURALLY OCCURING AMINO ACIDS, WHEREBY THERE IS PRODUCED AS ALPHA-(N-TERTIARY BUTYLOXY CARBONYL) TRIPEPTIDE LOWER ALKYL ESTER.

Claim 4

A PROCESS FOR PREPARING AN ALPHA-(N-TERTIARY BUTYLOXY CARBONYL) TRIPEPTIDE LOWER ALKYL ESTER COMPRISING THE STEPS OF (A) REACTING A MEMBER OF THE GROUP CONSISTING OF ALPHA-N-TERTIARYBUTYLOXYCARBONYL) -NATURALLY OCCURRIING AMINO ACIDS WITH A MEMBER SELECTED FROM THE GROUP CONSISTING OF LOWER ALKYL ESTER OF ALPHA NATURALLY OCCURING AMINO ACIDS IN THE PRESENCE OF A PEPTIDE REACTION-PROMOTING AGENT CONSISTING OF A LOWER ALKYL PYROPHOSPHITE AND A PHOSPHITE HYDROGEN HALIDE ACCEPTOR SELECTED FROM THE GROUP CONSISTING OF DI-)LOWER ALKYL PHOSPHITES, TRI-(LOWER ALKYL) PHOSPHITES, TRI-(LOWER ALKENYL) PHOSPHITES HAVING ALLYLIC UNSATURATION AND LOWER ALKYL ALKYLENE PHOSPHITES; (B) SUBJECTING THE PRODUCT OF SAID STEP (A), WHILE SAID PRODUCT IS STILL IN THE PEPTIDE-FORMING MEDIUM OF SAID STEP (A), TO THE ACTION OF A HYDROGEN HALIDE SELECTED FROM THE GROUP CONSISTING OF HYDROGEN BROMIDE AND HYDROGEN CHLORIDE WHEREBY THE TERTIARY BUTYLOXYCARBONYL ALPHA N-BLOCKING GROUP IS REMOVED; AND (C) REACTING THE PRODUCT OF SAID STEP (B), WHILE SAID PRODUCT OF SAID STEP (B) IS STILL IN THE REACTION MEDIUM OF SAID STEP (B), WITH A MEMBER OF THE GROUP CONSISTING OF ALPHA-(N-TERTIARY BUTYLOXY CARBONYL)NATURALLY OCCURING AMINO ACIDS, WHEREBY THERE IS PRODUCED AS ALPHA-(N-TERTIARY BUTYLOXY CARBONYL) TRIPEPTIDE LOWER ALKYL ESTER.