US3066079A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for purifying plasminogen
Assignee: Wyeth Holdings LLC
•
Filing: 1959-09-18
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Granted: 1962-11-27
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Expires: 1979-09-18
Abstract
No abstract record available.
Inventors
- 👤 James J Hagan
- 👤 Frank B Ablondi
- 👤 Ralph E Clarke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:27
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/185; A61K31/19; A61K31/195; C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6435; C12Y; C12Y304/00; C12Y304/21; C12Y304/21007; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
163 words
IN A PROCESS OF PURIFYING PLASMINOGEN WHICH COMPRISES SLURRYING PLASMA FRACTION III WITH DILUTE SULFURIC ACID AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A FIRST EXTRACT, ADJUSTING THE PH OF THE FIRST EXTRACT FIRST TO 11 FOR NO MORE THAN 3 MINUTES AND THEN TO 5.3 WITH COOLING FOR NO LESS THAN 3 HOURS, ADJUSTING THE PH OF THE RESULTING SUSPENSION TO 2 AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A SECOND EXTRACT, ADJUSTING THE PH OF THE SECOND EXTRACT TO 8.6 AND DIALYZING AGAINST 0.25 M SODIUM PHOSPHATE, COOLING THE RESULTING SUSPENSION AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A PLASMINOGEN CONCENTRATE; THE IMPROVEMENT WHICH COMPRISES ADJUSTING THE PH OF SAID PLASMINOGEN CONCENTRATE TO 5.5, ADDING TO THE RESULTING SUSPENSION A WATER SOLUTION OF 0.05TO 0.18 MOLAR CONCENTRATION OF A LYSINE COMPOUND SELECTED FROM THE GROUP CONSISTING OF LYSINE, LYSINE SALTS AND LYSINE ESTERS, SEPARATING SOLIDS FROM THE SUSPENSION AND CONCENTRATING THE SO OBTAINED EXTRACT TO A DRY POWER.
Claim 2
Dependent Claim
163 words
IN A PROCESS OF PURIFYING PLASMINOGEN WHICH COMPRISES SLURRYING PLASMA FRACTION III WITH DILUTE SULFURIC ACID AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A FIRST EXTRACT, ADJUSTING THE PH OF THE FIRST EXTRACT FIRST TO 11 FOR NO MORE THAN 3 MINUTES AND THEN TO 5.3 WITH COOLING FOR NO LESS THAN 3 HOURS, ADJUSTING THE PH OF THE RESULTING SUSPENSION TO 2 AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A SECOND EXTRACT, ADJUSTING THE PH OF THE SECOND EXTRACT TO 8.6 AND DIALYZING AGAINST 0.25 M SODIUM PHOSPHATE, COOLING THE RESULTING SUSPENSION AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A PLASMINOGEN CONCENTRATE; THE IMPROVEMENT WHICH COMPRISES ADJUSTING THE PH OF SAID PLASMINOGEN CONCENTRATE TO 5.5, ADDING TO THE RESULTING SUSPENSION A WATER SOLUTION OF 0.05TO 0.18 MOLAR CONCENTRATION OF A LYSINE COMPOUND SELECTED FROM THE GROUP CONSISTING OF LYSINE, LYSINE SALTS AND LYSINE ESTERS, SEPARATING SOLIDS FROM THE SUSPENSION AND CONCENTRATING THE SO OBTAINED EXTRACT TO A DRY POWER.
Claim 3
Dependent Claim
129 words
IN A PROCESS OF PURIFYING PLASMINOGEN WHICH COMPRISES SLURRYING PLASMA FRACTION III WITH DILUTE SULFURIC ACID AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A FIRST EXTRACT, ADJUSTING THE PH OF THE FIRST EXTRACT FIRST TO 11 FOR NO MORE THAN 3 MINUTES AND THEN TO 5.3 WITH COOLING FOR LESS THAN 3 HOURS, ADJUSTING THE PH OF THE RESULTING SUSPENSION TO 2 AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A SECOND EXTRACT, ADJUSTING THE PH OF THE SECOND EXTRACT TO 8.6 AND DIALYZING AGAINST 0.25 M SODIUM PHOSPHATE, COOLING THE RESULTING SUSPENSION AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRDUCING A PLASMINOGEN CONCENTRATE; THE IMPROVEMENT WHICH COMPRISES ADSORBING SAID PLASMINOGEN CONCENTRATE ON CARBOXYMETHYL CELLULOSE AT A PH OF 2.5 TO 3.5 AND ELUTING WITH AN AQUEOUS ACID.
Claim 4
Dependent Claim
129 words
IN A PROCESS OF PURIFYING PLASMINOGEN WHICH COMPRISES SLURRYING PLASMA FRACTION III WITH DILUTE SULFURIC ACID AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A FIRST EXTRACT, ADJUSTING THE PH OF THE FIRST EXTRACT FIRST TO 11 FOR NO MORE THAN 3 MINUTES AND THEN TO 5.3 WITH COOLING FOR LESS THAN 3 HOURS, ADJUSTING THE PH OF THE RESULTING SUSPENSION TO 2 AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRODUCING A SECOND EXTRACT, ADJUSTING THE PH OF THE SECOND EXTRACT TO 8.6 AND DIALYZING AGAINST 0.25 M SODIUM PHOSPHATE, COOLING THE RESULTING SUSPENSION AND SEPARATING SOLIDS FROM THE SUSPENSION THEREBY PRDUCING A PLASMINOGEN CONCENTRATE; THE IMPROVEMENT WHICH COMPRISES ADSORBING SAID PLASMINOGEN CONCENTRATE ON CARBOXYMETHYL CELLULOSE AT A PH OF 2.5 TO 3.5 AND ELUTING WITH AN AQUEOUS ACID.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1965-11-26 | 1966-05-28 | 1966-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1969-11-26 | 1970-05-28 | 1970-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1973-11-26 | 1974-05-28 | 1974-11-27 | $7700.00 | Window Closed / Lapsed |