US3049530A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Soybean trypsin inhibitors and method of isolating the same
Assignee: Individual
•
Filing: 1959-08-25
•
Granted: 1962-08-14
•
Expires: 1979-08-25
Abstract
No abstract record available.
Inventors
- 👤 Joseph J Rackis
- 👤 Allan K Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:01
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/55; A61K38/57; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
151 words
A METHOD FOR OBTAINING SOYBEAN TRYPSIN INHIBITOR FRACTIONS FROM SOYBEAN WHEY WHICH COMPRISES PROVIDING A DIETHYLAMINOETHYL CELLULOSE CHROMATOGRAPHY COLUMN BUFFER-EQUILIBRATED WITH 0.01 M PHOSPHATE TO PH 7.6 PASSING SOYBEAN WHEY THROUGH THE COLUMNN TO CAUSE PROTEINS IN THE WHEY TO BE ABSORBED ON SAID COLUMN; STEPWISE ELUTING THE COLUMN FIRST WITH 0.034M SODIUM CHLORIDE IN 0.01 M PHOSPHATE BUFFER AND THEN WITH 0.13 M SODIUM CHLORIDE IN SAID BUFFER TO REMOVE UNWANTED COMPONENTS OF THE WHEY FROM THE COLUMN; NEXT ELUTING WITH 0.17 M SODIUM CHLORIDE IN SAID BUFFER, APPROXIMATELY THE FIRST HALF OF THE CORRESPONDING EFFLUENT BEING DISCARDED WHILE RETAINING THE REMAINING FRACTION; THEN ELUTING THE COLUMN WITH 0.25M SODIUM CHLORIDE IN SAID BUFFER, APPROXIMATELY THE FIRST HALF OF THE CORRESPONDING EFFLUENT BEING DISCARDED WHILE RETAINING THE REMAINING FRACTION; SEPARATELY DIALYZING THE SAID RETAINED FRACTIONS AGAINST DISTILLED WATER, AND SEPARATELY LYOPHILIZING THE DIALYZED FRACTION TO RECOVER SOYBEAN TRYPSIN INHIBITOR FRACTIONS.
Claim 2
Independent Claim
151 words
A METHOD FOR OBTAINING SOYBEAN TRYPSIN INHIBITOR FRACTIONS FROM SOYBEAN WHEY WHICH COMPRISES PROVIDING A DIETHYLAMINOETHYL CELLULOSE CHROMATOGRAPHY COLUMN BUFFER-EQUILIBRATED WITH 0.01 M PHOSPHATE TO PH 7.6 PASSING SOYBEAN WHEY THROUGH THE COLUMNN TO CAUSE PROTEINS IN THE WHEY TO BE ABSORBED ON SAID COLUMN; STEPWISE ELUTING THE COLUMN FIRST WITH 0.034M SODIUM CHLORIDE IN 0.01 M PHOSPHATE BUFFER AND THEN WITH 0.13 M SODIUM CHLORIDE IN SAID BUFFER TO REMOVE UNWANTED COMPONENTS OF THE WHEY FROM THE COLUMN; NEXT ELUTING WITH 0.17 M SODIUM CHLORIDE IN SAID BUFFER, APPROXIMATELY THE FIRST HALF OF THE CORRESPONDING EFFLUENT BEING DISCARDED WHILE RETAINING THE REMAINING FRACTION; THEN ELUTING THE COLUMN WITH 0.25M SODIUM CHLORIDE IN SAID BUFFER, APPROXIMATELY THE FIRST HALF OF THE CORRESPONDING EFFLUENT BEING DISCARDED WHILE RETAINING THE REMAINING FRACTION; SEPARATELY DIALYZING THE SAID RETAINED FRACTIONS AGAINST DISTILLED WATER, AND SEPARATELY LYOPHILIZING THE DIALYZED FRACTION TO RECOVER SOYBEAN TRYPSIN INHIBITOR FRACTIONS.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1965-08-13 | 1966-02-12 | 1966-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1969-08-13 | 1970-02-12 | 1970-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1973-08-13 | 1974-02-12 | 1974-08-14 | $7700.00 | Window Closed / Lapsed |