Method of making dextrose using purified amyloglucosidase

Claim 1

IN A PROCESS FOR PRODUCING DEXTROSE FROM STARCH WHEREIN THE STARCH IN AQUEOUS SYSTEM IS HYDORLYZED WITH AN AMYLOGLCOSIDASE CONTAINING ENZYME PREPARATION TO MAXIMAL D.E. AND TRUE DEXTROSE CONTENTS, THE HYDORLYZATE IS CLARIFIED, CONCENTRATED BY EVAPORATION, AND ALLOWED TO CRYSTALLIZE, AND THE CRYSTALLIZED DEXTOSE IS SEPARATED FROM THE MOTHER LIQUOR, THE IMPROVEMENT WHICH CONSISTS IN HYDROLYZING THE STARCH WITH AN AMYLOGLUCOSIDASE CONTAINING ENZYME PREPARATION WHICH HAS BEEN PUIFIED BY DEVELOPING UPON A CELLULOSIC BED A CHROMATOGRAM FROM AN AQUEOUS SOLUTION THAT CONTAINS AMYLOGLUCOSIDASE, AND USING AS A DEVELOPING AGENT A LIQUID THAT IS A SOLUTION IN WATER OF A WATER-MISCIBLE NON-SOLVENT FOR THE ENZYME, AND THEREAFTER ELUTING FROM THE CHROMATOGRAM THE PORTION THEREOF CONSISTING ESSENTIALLY OF THE AMYLOGLUCOSIDASE IN PURIFIED FORM; SAID HYDROLYSIS RESULTING IN A FINAL HYDROLYZATE WHICH HAS MAXIMAL D.E. VALUES OF ABOUT 93 TO 96 PERCENT AND MAXIMAL TRUE DEXTROSE CONTENTS FO ABOUT 87 TO 94 PERCENT, DRY BASIS.