US2982696A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Ion-exchange procedures for the purification of penicillinase
Assignee: Schenley Industries Inc
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Filing: 1959-05-01
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Granted: 1961-05-02
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Expires: 1979-05-01
Abstract
No abstract record available.
Inventors
- 👤 Puetzer Bruno
- 👤 Boschetti Armando
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 10:09
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/86; B; B01; B01J; B01J47/00; B01J47/014; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
97 words
PROCESS FOR RECOVERING PENICILLINASE FROM RELATIVELY CRUDE SOLUTIONS OF THE ENZYME THAT COMPRISES, CONTACTING A RELATIVELY IMPURE SOLUTION OF PENICILLINASE WITH THE CATION EXCHANGE RESIN COMPOSED OF A CROSS-LINKED COPOLYMER OF METHACRYLIC ACID AND DIVINYL BENZENE, CONTAINING BETWEEN 4 AND 10 MOL PERCENT OF DIVINYL BENZENE BUFFERED TO APPROXIMATELY PH 5.5 TO EFFECT SELECTIVE ADSORPTION OF PENICILLINASE ONTO SAID EXCHANGE RESIN, ELUTING THE ADSORBED PENICILLINASE FROM SAID EXCHANGE RESIN BY MEANS OF AN AQUEOUS SOLUTION CONTAINING APPROXIMATELY 10% BY WEIGHT OF SODIUM CITRATE, AND DIALYZING SAID SODIUM CITRATE-PENICILLINASE SOLUTION TO PROVIDE A PURIFIED SOLUTION OF THE ENZYME.
Claim 2
Dependent Claim
97 words
PROCESS FOR RECOVERING PENICILLINASE FROM RELATIVELY CRUDE SOLUTIONS OF THE ENZYME THAT COMPRISES, CONTACTING A RELATIVELY IMPURE SOLUTION OF PENICILLINASE WITH THE CATION EXCHANGE RESIN COMPOSED OF A CROSS-LINKED COPOLYMER OF METHACRYLIC ACID AND DIVINYL BENZENE, CONTAINING BETWEEN 4 AND 10 MOL PERCENT OF DIVINYL BENZENE BUFFERED TO APPROXIMATELY PH 5.5 TO EFFECT SELECTIVE ADSORPTION OF PENICILLINASE ONTO SAID EXCHANGE RESIN, ELUTING THE ADSORBED PENICILLINASE FROM SAID EXCHANGE RESIN BY MEANS OF AN AQUEOUS SOLUTION CONTAINING APPROXIMATELY 10% BY WEIGHT OF SODIUM CITRATE, AND DIALYZING SAID SODIUM CITRATE-PENICILLINASE SOLUTION TO PROVIDE A PURIFIED SOLUTION OF THE ENZYME.
References Cited (Backward Citations)
US2886489A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1964-05-01 | 1964-10-31 | 1965-05-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1968-05-01 | 1968-10-31 | 1969-05-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1972-05-01 | 1972-10-31 | 1973-05-02 | $7700.00 | Window Closed / Lapsed |