Manufacture of polyoxygenated dehydro-steroids

Claim 1

IN A METHOD FOR DEHYDROGENATING THE 1:2-POSITION AND OXYGENATING AT LEAST ONE OF THE POSITIONS 11, 17 AND 21 IN $4-3:20-DIOXO-PREGNENE, SATURATED IN THE 1:2-POSITIONS TION AND NONOXYGENATED IN AT LEAST ONE OF THE POSITIONS 11, 17 AND 21, THE STEP WHICH COMPRISES SUBJECTING A $4-3:20-DIOXO-PREGNENE, SATURATED IN THE 1:2-POSITION AND NON-OXYGENATED IN AT LEAST ONE OF THE POSITIONS 11, 17 AND 21, SIMULTANEOUSLY TO ENZYMES FROM AEROBIC CULTURES OF FUNGI SELECTED FROM THE GROUP CONSISTING OF CALONECTRIA DECORA, OPHIOBOLUS HETEROSTROPHUS, OPHIOBOLUS MIYABEANUS, ALTERNARIA PASSIFLORAE AND DIDYMELLA LYCOPERSICI FOR DEHYDROGENATION OF THE 1:2-POSITION, AND TO AT LEAST ONE TYPE OF ENZYMES PRODUCED FROM ONE OF THE THREE GROUPS OF FUNGI CONSISTING OF (A) THE GENERA RHIZOPUS, MUCOR, ASPERGILUS, PENICILLIUM, CURVULARIA, CUNNINGHAMELLA, SPONDYLOCLADIUM AND STREPTOMYCES FOR OXIDATION IN POSITION 11, (B) THE SPECIES TRICHOTHECIUM ROSEUM, LEPTOSPHAERIA MACULANS, CUCURBITARIA LABURNI, ACROSPEIRA LEVIS, LOPHOTRICHUS MARTINII, MELANOSPORA PARASITICA AND THIELAVIA TERRICOLA FOR OXIDATION IN POSITION 17 AND (C) THE SPECIES OPHIOBOLUS HERPORTICHUS AND SCLEROTINIA FRUCTICOLA FOR OXIDATION IN POSITION 21, SAID TREATMENT BEING CARRIED OUT IN A SINGLE OPERATION.