US2957809A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Insulinase
Assignee: Merck and Co Inc
•
Filing: 1959-04-13
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Granted: 1960-10-25
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Expires: 1979-04-13
Abstract
No abstract record available.
Inventors
- 👤 Norman G Brink
- 👤 Urban J Lewis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 02:24
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6489; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
159 words
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
Claim 2
Dependent Claim
159 words
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
References Cited (Backward Citations)
US2803585A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1963-10-25 | 1964-04-25 | 1964-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1967-10-25 | 1968-04-25 | 1968-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1971-10-25 | 1972-04-25 | 1972-10-25 | $7700.00 | Window Closed / Lapsed |