Kanamycin and processes for the preparation thereof

Claim 1

AN ANTIBIOTIC SUBSTANCE EFFECTIVE IN INHIBITING THE GROWTH OF GRAM-POSITIVE BACTERIA, GRAM-NEGATIVE BACTERIA AND MYCOBACTERIA SELECTED FROM THE GROUP CONSISTING OF KANAMYCIN A AND KANAMYCIN B AND ACID ADDITION SALTS THEREOF, EACH OF SAID KANAMYCINS BEING A SUBSTANCE WHICH IS SOLUBLE IN WATER AND SUBSTANTIALLY INSOLUBLE IN N-BUTANOL, ETHYL ACETATE, BUTYL ACETATE, ETHER, CHLOROFORM AND BENZENE, WHICH FORMS SALTS WITH ACIDS, WHICH EXHIBITS NO ABSORPTION OF ULTRAVIOLET LIGHT FROM 220 MU TO 400 MU, WHICH GIVES A POSITIVE REACTION TO NINHYDRIN REAGENT DISSOLVED IN PYRIDINE AND POSITIVE MOLISCH AND ELSON-MORGAN REACTIONS AND NEGATIVE TOLLENS, SAKAGUCHI, FEHLING, MALTOL AND SELIWANOFF REACTIONS, WHICH CONTAINS ONLY THE ELEMENTS CARBON, HYDROGEN, OXYGEN AND NITROGEN, WHICH CONTAINS FREE PIMARY AMINO GROUPS, WHICH IS DEXTRO-ROTATORY IN WATER AND WHICH EXHIBITS CHARACTERISTIC ABSORPTION BANDS IN THE INFRA-RED REGION OF THE SPECTRUM WHEN PELLETED IN THE FORM OF THE FREE BASE IN POTASSIUM BROMIDE AT THE FOLLOWING WAVE LENGTHS IN MICRONS: 2.96, 6.35, 6.48, 6.85, 7.25, 7.45, 7.86, 8.08, 9.65 AND 10.4, FURTHER PROPERTIES OF SAID KANAMYCINS BEING THAT KANAMYCIN A BASE HAS THE EMPIRICAL FORMULA C18H34-36N4O11, EXHIBITS (A)D24 +146* (C=1, 0.1 NH2SO4), GIVES A SALICYLIDENE DERIVATIVE MELTING A 272274*C. WITH DECOMPOSITION, GIVES DESOXYSTREPTAMINE ON STRONG ACID HYDROLYSIS, GIVES A PRODUCT WITH AN ULTRAVIOLET ABSORTION SPECTRUM IDENTICAL TO THAT OF FURFURAL ON TREATMENT WITH 40% SULFURIC ACID FOR 100 MINUTES AT 100*C. AND EXHIBITS ADDITIONAL CHARACTERISTIC ABSORPTION BANDS IN THE INFRA-RED REGION OF THE SPECTRUM WHEN PELLETED IN THE FORM OF THE FREE BASE IN POTASSIUM BROMIDE AT THE FOLLOWING WAVE LENGTHS IN MICRONS: 2.86, 2.93, 3.01, 3.03, 3.15, 3.22, 3.67, 6.23, 6.75, 6.93, 7.10, 7.33, 7.57, 7.63, 7.75, 7.82, 7.90, 7.95, 8.19, 8.43, 8.55, 8.68, 8.79, 8.90, 9.00, 9.20, 9.47, 9.85, 10.08, 10.63, 10.95, 11.25, 11.35, 11.50, 11.73, 11.95, 12.28, 12.48, 12.77 AND 13.05, AND THAT KANAMYCIN B BASE EXHIBITS (A)D+135* (C=0.63 IN WATER), GIVES A SALICYLIDENE DERIVATIVE WHICH DECOMPOSES WITHOUT MELTING AT 255-265*C., FAILS TO GIVE A PRODUCT WITH AN ULTRAVIOLET ABSORPTION SPECTRUM IDENTICAL TO THAT OF FURFURAL ON TREATMENT WITH 4/% SULFURIC ACID FOR 100 MINUTES AT 100*C. AND EXHIBITS ADDITIONAL CHARACTERISTIC ABSORPTION BANDS IN THE INFRA-RED REGION OF THE SPECTRUM WHEN PELLETED IN THE FORM OF THE FREE BASE IN POTASSIUM BROMIDE AT THE FOLLOWING WAVE LENGTHS IN MICRONS: 3.44, 6.74, 8.28, 8.76, 9.55 AND 11.15.