US2809148A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for purifying and separating b12-group-vitamins by partition chromatography on cellulose columns
Assignee: Aschaffenburger Zellstoffwerke AG
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Filing: 1954-12-07
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Granted: 1957-10-08
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Expires: 1974-12-07
Abstract
No abstract record available.
Inventors
- 👤 Bernhauer Konrad
- 👤 Friedrich Wilhelm
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 05:36
CPC Classifications
C; C07; C07H; C07H23/00; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
79 words
A PROCESS FOR PURIFYING AND SEPARATING B12 GROUP VITAMINS BY MEANS OF PARTITION CHROMATOGRAPHY, COMPRISING THE STEPS OF PREPARING A PULP FROM A CELLULOSE MATERIAL AND A STRAIGHT CHAIN BUTYL ALCOHOL CONTAINING WATER, CHARGING THE PULP ONTO A CHROMATOGRAPHIC COLUMN, THEN CHARGING A MIXTURE OF B12 VITAMIN FACTORS ONTO THE COLUMN, AND DEVELOPING THE CHROMATOGRAM BY ADDING A WATER-CONTAINING DEVELOPER COMPRISING A STRAIGHT CHAIN BUTYL ALCOHOL, THEREBY ELUTING EACH OF THE VITAMIN B12 FACTORS CONTAINED IN SAID MIXTURE SEPARATELY.
Claim 2
Dependent Claim
79 words
A PROCESS FOR PURIFYING AND SEPARATING B12 GROUP VITAMINS BY MEANS OF PARTITION CHROMATOGRAPHY, COMPRISING THE STEPS OF PREPARING A PULP FROM A CELLULOSE MATERIAL AND A STRAIGHT CHAIN BUTYL ALCOHOL CONTAINING WATER, CHARGING THE PULP ONTO A CHROMATOGRAPHIC COLUMN, THEN CHARGING A MIXTURE OF B12 VITAMIN FACTORS ONTO THE COLUMN, AND DEVELOPING THE CHROMATOGRAM BY ADDING A WATER-CONTAINING DEVELOPER COMPRISING A STRAIGHT CHAIN BUTYL ALCOHOL, THEREBY ELUTING EACH OF THE VITAMIN B12 FACTORS CONTAINED IN SAID MIXTURE SEPARATELY.
Claim 3
Dependent Claim
119 words
A PROCESS FOR PURIFYING AND SEPARATING B12 GROUP VITAMINS BY MEANS OF PARTITION CHROMATOGRAPHY, COMPRISING THE STEPS OF PREPARING A PULP FROM CELLULOSE MATERIAL AND A STRAIGHT-CHAIN BUTYL ALCOHOL CONTAINING WATER, CHARGING THE PULP ONTO A CHROMATOGRAPHIC COLUMN, THEN CHARGING A KIESELGUHR PRODUCT OF A MIXTURE OF B12 VITAMIN FACTORS ONTO THE COLUMN, AND THEREAFTER DEVELOPING THE CHROMATOGRAM BY ADDING A DEVELOPER CONTAINING WATER, A STRAIGHT CHAIN BUTYL ALCOHOL, AND AT LEAST ONE EASILY DISSOCIABLE SALT HAVING A MONOBASIC ANION CONSISTING OF AT LEAST FOUR NON-METAL ATOMS, AND A MONOVALENT CATION SELECTED FROM THE GROUP CONSISTING OF SODIUM AND POTASSIUM, THEREBY IMPROVING THE DISPERSION OF THE CHROMATOGRAM AND ELUTING EACH OF THE VITAMIN B12 FACTORS SEPARATELY FROM SAID MIXTURE.
Claim 4
Dependent Claim
119 words
A PROCESS FOR PURIFYING AND SEPARATING B12 GROUP VITAMINS BY MEANS OF PARTITION CHROMATOGRAPHY, COMPRISING THE STEPS OF PREPARING A PULP FROM CELLULOSE MATERIAL AND A STRAIGHT-CHAIN BUTYL ALCOHOL CONTAINING WATER, CHARGING THE PULP ONTO A CHROMATOGRAPHIC COLUMN, THEN CHARGING A KIESELGUHR PRODUCT OF A MIXTURE OF B12 VITAMIN FACTORS ONTO THE COLUMN, AND THEREAFTER DEVELOPING THE CHROMATOGRAM BY ADDING A DEVELOPER CONTAINING WATER, A STRAIGHT CHAIN BUTYL ALCOHOL, AND AT LEAST ONE EASILY DISSOCIABLE SALT HAVING A MONOBASIC ANION CONSISTING OF AT LEAST FOUR NON-METAL ATOMS, AND A MONOVALENT CATION SELECTED FROM THE GROUP CONSISTING OF SODIUM AND POTASSIUM, THEREBY IMPROVING THE DISPERSION OF THE CHROMATOGRAM AND ELUTING EACH OF THE VITAMIN B12 FACTORS SEPARATELY FROM SAID MIXTURE.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1960-10-07 | 1961-04-08 | 1961-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1964-10-07 | 1965-04-08 | 1965-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1968-10-07 | 1969-04-08 | 1969-10-08 | $7700.00 | Window Closed / Lapsed |