ph control in production of fumagillin

Claim 1

IN A PROCESS WHICH COMPRISES ACEROBICALLY FERMENTING AN AQUEOUS ANTIBIOTIC NUTRIENT MEDIUM CONTAINING AN ADEQUATE SOURCE OF CARBON, NITROGEN, AND NECESSARY MINERALS AND INOCULATED WITH A CULTURE OF A FUMAGILLIN PRODUCING STRAINS OF ASPERGILLUS FUMIGATUS AND RECOVERING THE FUMAGILLIN PRODUCED THEREFROM, THE IMPROVED STEP COMPRISING; ADDING TO THE FERMENATION MEDIUM DURING THE FERMENTATION PROCESS WHEN THE PH OF THE SAID MEDIUM HAS A VALUE IN EXCESS OF ABOUT PH7 AND BEFORE THE PH THE SAID MEDIUM IS APPREABLY IN EXCESS OF ABOUT PH 7.5 A SMALL AMOUNT OF AN ACID PRODUCING CARBOHYDRATE ADAPTED TO BEING OXIDIZED DURING THE SAID FERMENTATION BY THE SAID CULTURE TO FORM ACIDIC PRODUCTS WHICHH MAINTAIN THE PH BELOW A MAXIMUM PH OF 7.5 DURING THE COURSE OF THE FERMENTATION PROCESS; WHEREBY THE NORMAL INCREASE IN ALKALINITY OF THE FERMENTATION MEDIUM IS INHIBITED AND THE ADVERSE EFFECT OF ALKALINE CONDITION UPON THE FUMAGILLIN FERMENTATION YIELD IS OVERCOME.