Method of purifying and isolating proteins

Claim 1

THE METHOD OF SEPARATING PROTEIN FROM NON-PROTEINACEOUS MATTER IN SOLUTIONS WHICH COMPRISES ADMIXING WITH AN AQUEOUS SOLUTION OF THE PROTEIN AND NON-PROTEINACEOUS IMPURITIES A WATER-SOLUBLE SALT OF A HIGH MOLECULAR WEIGHT AMINE, WHICH AMINE IN THE FREE STATE IS INSOLUBLE IN WATER, TO FORM A SOLUTION CONTAINING THE PROTEIN AND THE AMINE SALT AT AN ACID PH AT WHICH THE AMINE SALT IS IN SOLUTION, SAID AMINE BEING SELECTED FROM THE GROUP WHICH CONSISTS OF N,N-DIMETHYL-N''-(2-PYRIDYL)-N''(2-THIENYLMETHYL) - ETHYLENEDIAMINE; N,N - DIMETHYL-N''PHENYL-N''-(5-CHLORO-2-THIENYLMETHYL) - ETHYLENEDIAMINE; N,N - DIMETHYL-N''-PHENYL-N''-(2-THIENYLMETHYL)-ETHYLENEDIAMINE; N,N - DIMETHYL-N''-(2-PYRIDYL)-N'' - (5-CHLORO-2THIENYLMETHYL)-ETHYLENEDIAMINE; A-DIMETHYLAMINOMETHYLA-PHENYL - P - METHOXY-ACETOPHENONE; 1,5-BIS (B-DIETHYLAMINO-METHOXY) - NAPHTHALENE; 1-(B-DIETHYLAMINO-METHOXY)-2-CHLORO-4-TERT. BUTYLBENZENE; 10-(DIMETHYL-AMINOPROPYL) PHENOTHIAZINE; 2-METHYL-9-PHENYL-2,3,4,9-TETRAHYDRO-1-PYRIDINDENE; 1-(3,4-DIMETHYLPHENYL)-2-AMINO PROPANE; 2,7-DIMETHYL-2,3,4,9-TETRA HYDRO-9-(P-TOLYL)-1-PYRIDINDENE; 2,6-DIMETHYL-4-PHENYLPIPERIDINE; B-DIMETHYLAMINOETHYL BENZHYDRYL ETHER; 2-(N-BENZYL-N-PHENYL-AMINOMETHYL) - IMIDAZOLINE; 3,6-DIAMINO-10-METHYLACRIDINIUM; 2,8-DIAMINOACRIDINE; 3,6 - BIS (DIMETHYLAMINO) - XANTHYL LUM; AND N,N-DIMETHYL-N''-(A-PYRIDYL)-N''-BENZYLETHYLENEDIAMINE, ADJUSTING THE PH OF THE SOLUTION TO APPROXIMATE NEUTRALITY AND THEREBY PRECIPITATING THE INSOLUBLE AMINE IN THE FORM OF AN AMINE-PROTEIN COMPLEX, SEPARATING THE PRECIPITATED COMPLEX FROM THE SOLUTION, AND TREATING THE COMPLEX WITH A SOLVENT SELECTED FROM THE CLASS WHICH CONSISTS OF WATER AND SALINE SOLUTIONS AT A SLIGHTLY ALKALINE PH AND LOWER ALIPHATIC ALCOHOLS WHICH DISSOLVES ONE OF THE CONSTITUENTS OF THE AMINE-PROTEIN COMPLEX TO SEPARATE THE PROTEIN FROM THE AMINE.

Claim 2

THE METHOD OF SEPARATING PROTEIN FROM NON-PROTEINACEOUS MATTER IN SOLUTIONS WHICH COMPRISES ADMIXING WITH AN AQUEOUS SOLUTION OF THE PROTEIN AND NON-PROTEINACEOUS IMPURITIES A WATER-SOLUBLE SALT OF A HIGH MOLECULAR WEIGHT AMINE, WHICH AMINE IN THE FREE STATE IS INSOLUBLE IN WATER, TO FORM A SOLUTION CONTAINING THE PROTEIN AND THE AMINE SALT AT AN ACID PH AT WHICH THE AMINE SALT IS IN SOLUTION, SAID AMINE BEING SELECTED FROM THE GROUP WHICH CONSISTS OF N,N-DIMETHYL-N''-(2-PYRIDYL)-N''(2-THIENYLMETHYL) - ETHYLENEDIAMINE; N,N - DIMETHYL-N''PHENYL-N''-(5-CHLORO-2-THIENYLMETHYL) - ETHYLENEDIAMINE; N,N - DIMETHYL-N''-PHENYL-N''-(2-THIENYLMETHYL)-ETHYLENEDIAMINE; N,N - DIMETHYL-N''-(2-PYRIDYL)-N'' - (5-CHLORO-2THIENYLMETHYL)-ETHYLENEDIAMINE; A-DIMETHYLAMINOMETHYLA-PHENYL - P - METHOXY-ACETOPHENONE; 1,5-BIS (B-DIETHYLAMINO-METHOXY) - NAPHTHALENE; 1-(B-DIETHYLAMINO-METHOXY)-2-CHLORO-4-TERT. BUTYLBENZENE; 10-(DIMETHYL-AMINOPROPYL) PHENOTHIAZINE; 2-METHYL-9-PHENYL-2,3,4,9-TETRAHYDRO-1-PYRIDINDENE; 1-(3,4-DIMETHYLPHENYL)-2-AMINO PROPANE; 2,7-DIMETHYL-2,3,4,9-TETRA HYDRO-9-(P-TOLYL)-1-PYRIDINDENE; 2,6-DIMETHYL-4-PHENYLPIPERIDINE; B-DIMETHYLAMINOETHYL BENZHYDRYL ETHER; 2-(N-BENZYL-N-PHENYL-AMINOMETHYL) - IMIDAZOLINE; 3,6-DIAMINO-10-METHYLACRIDINIUM; 2,8-DIAMINOACRIDINE; 3,6 - BIS (DIMETHYLAMINO) - XANTHYL LUM; AND N,N-DIMETHYL-N''-(A-PYRIDYL)-N''-BENZYLETHYLENEDIAMINE, ADJUSTING THE PH OF THE SOLUTION TO APPROXIMATE NEUTRALITY AND THEREBY PRECIPITATING THE INSOLUBLE AMINE IN THE FORM OF AN AMINE-PROTEIN COMPLEX, SEPARATING THE PRECIPITATED COMPLEX FROM THE SOLUTION, AND TREATING THE COMPLEX WITH A SOLVENT SELECTED FROM THE CLASS WHICH CONSISTS OF WATER AND SALINE SOLUTIONS AT A SLIGHTLY ALKALINE PH AND LOWER ALIPHATIC ALCOHOLS WHICH DISSOLVES ONE OF THE CONSTITUENTS OF THE AMINE-PROTEIN COMPLEX TO SEPARATE THE PROTEIN FROM THE AMINE.