US2677642A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of enzymes
Assignee: Wyeth Holdings LLC
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Filing: 1951-08-03
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Granted: 1954-05-04
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Expires: 1971-08-03
Abstract
No abstract record available.
Inventors
- 👤 Frank B Ablondi
- 👤 Nalesnyk Stephen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 13:03
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/16; C07; C07K; C07K14/00; C07K14/195; C07K14/315; C07K14/3153; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
91 words
A METHOD OF PURIFYING ENZYMES SELECTED FROM THE GROUP CONSISTING OF KINASES ANDD DESOXYRIBONUCLEASES PRODUCED BY THE FERMENTATION OF MICROORGANISMS WHICH METHOD COMPRISES TREATING THE ENZYME MATERIAL WITH .005 TO 1.5 PARTS BY WEIGHT OF PROTAMINE PER PART BY WEIGHT OF TOTAL NITROGEN IN SAID ENZYME MATERIAL, IN A 0.01 TO 1.0 MOLAR SOLUTION OF AN ALKALINE EARTH METAL CATION, AT A TEMPERATURE OF ABOUT 0*C. TO ABOUT 40* C. AND AT A HYDROGEN ION CONCENTRATION FROM ABOUT PH 5.0 TO ABOUT PH 8.0. AND REMOVING THE RESULTING PRECIPITATE OF IMPURITIES.
Claim 2
Independent Claim
91 words
A METHOD OF PURIFYING ENZYMES SELECTED FROM THE GROUP CONSISTING OF KINASES ANDD DESOXYRIBONUCLEASES PRODUCED BY THE FERMENTATION OF MICROORGANISMS WHICH METHOD COMPRISES TREATING THE ENZYME MATERIAL WITH .005 TO 1.5 PARTS BY WEIGHT OF PROTAMINE PER PART BY WEIGHT OF TOTAL NITROGEN IN SAID ENZYME MATERIAL, IN A 0.01 TO 1.0 MOLAR SOLUTION OF AN ALKALINE EARTH METAL CATION, AT A TEMPERATURE OF ABOUT 0*C. TO ABOUT 40* C. AND AT A HYDROGEN ION CONCENTRATION FROM ABOUT PH 5.0 TO ABOUT PH 8.0. AND REMOVING THE RESULTING PRECIPITATE OF IMPURITIES.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1957-05-03 | 1957-11-02 | 1958-05-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1961-05-03 | 1961-11-02 | 1962-05-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1965-05-03 | 1965-11-02 | 1966-05-04 | $7700.00 | Window Closed / Lapsed |