Isolation and purification of streptomycin phosphate

Claim 1

A PROCESS FOR REFINING STREPTOMYCIN PHOSPHATE WHICH COMPRISES THE STEPS OF ADDING ACTIVATED CARBON TO A FILTERED FERMENTATION BROTH CONTAINING STREPTOMYCIN WITH THE SUBSEQUENT ELUTION OF THE STREPTOMYCIN FROM THE ACTIVATED CARBON BY THE ADDITION OF PHOSPHORIC ACID, CONCENTRATING THE SUBSTANTIALLY NEUTRALIZED ELUATE UNDER REDUCED PRESSURE, TREATING THE CONCENTRATE WITH SUFFICIENT TERTIARY BUTYL ALCOHOL WITHIN THE RANGE OF 1 TO 10 VOLUMES TO CAUSE PRECIPITATION, SEPARATING THE PRECIPITATE, REDISSOLVING THE PRECIPITATE IN WATER TO A CONCENTRATION OF AT LEAST 100,000 UNITS-ML. ADJUSTING THE PH OF THIS SOLUTION TO ABOUT 6, ADDING ACTIVATED CARBON TO REMOVE PYROGENS, AND ADDING THE STREPTOMYCIN CONCENTRATE THUS OBTAINED TO AN ALCOHOL TO OBTAIN A HIGHLY PURIFIED PRECIPITATE OF STREPTOMYCIN PHOSPHATE.