Vitamin products and methods of obtaining the same

Claim 1

PROCESS FOR THE ISOLATION OF A COMPOUND HAVING CHICK ANTI-ANEMIA ACTIVITY AND SUBSTANTIALLY NO GROWTH EFFECT ON LACTOBACILLUS CASEI WHICH COMPRISES ADSORBING SAID ACTIVITY ON CHARCOAL FROM AN ACIDIFIED AQUEOUS EXTRACT CONTAINING THE CHICK ANTI-ANEMIA ACTIVITY, ELUTING THE ACTIVITY WITH AQUEOUS ALCOHOLIC AMMONIA, CONCENTRATING THE ELUATE AND EXTRACTING THE CONCENTRATE EXHAUSTIVELY WITH A LOWER ALIPHATIC ALCOHOL HAVING A SLIGHT MISCIBILITY WITH WATER, EVAPORATING THE AQUEOUS PHASE CONTAINING THE DESIRED ACTIVITY TO DRYNESS, TAKING THE RESIDUE UP IN WATER, ADDING THE RESULTANT SOLUTION TO A LOWER ALKYL KETONE ACIDIFIED WITH MINERAL ACID, FILTERING THE MIXTURE, ADSORBING THE ACTIVITY FROM THE FILTRATE ON CHARCOAL, ELUTING THE ACTIVITY WITH AQUEOUS ALCOHOLIC AMMONIA, CONCENTRATING THE ELUATE, EXTRACTING THE CONCENTRATE WITH PHENOL, SATURATING THE AQUEOUS PHASE CONTAINING THE CHICK ANTI-ANEMIA ACTIVITY WITH AMMONIUM SULFATE, EXTRACTING THE SOLUTION WITH PHENOL, TREATING THE PHENOL PHASE WITH ETHER AND WATER, ADJUSTING THE PH OF THE AQUEOUS PHASE WHICH SEPARATES TO ABOUT 7, PRECIPITATING THE ACTIVE PRINCIPLE FROM SAID AQUEOUS PHASE AS THE ZINC SALT, SUSPENDING THE ZINC SALT IN AN AQUEOUS ACID SOLUTION AT ABOUT PH 2.5, FILTERING THE MIXTURE, EXTRACTING THE FILTRATE WITH PHENOL, TREATING THE PHENOL PHASE WITH ETHER AND WATER, ADJUSTING THE PH OF THE RESULTANT AQUEOUS PHASE TO ABOUT 7, CONCENTRATING THE SOLUTION, PRECIPITATING THE ACTIVE PRINCIPLE FROM THE CONCENTRATE AS THE ZINC SALT, TREATING THE DRY ZINC SALT WITH LOWER ALKYL ALCOHOL AND A SMALL AMOUNT OF MINERAL ACID, EVAPORATING THE MIXTURE, ADDING WATER AND SEPARATING THE PRECIPITATED CRYSTALLINE LOWER ALKYL ESTER OF THE CHICK ANTI-ANEMIA FACTOR, HYDROLYZING THE LOWER ALKYL ESTER WITH ALKALI AND SEPARATING FROM THE HYDROLYSATE IN SUBSTANTIALLY PURE FORM THE VITAMIN CONJUGATE HAVING CHICK ANTIANEMIA VITAMIN ACTIVITY AND SUBSTANTIALLY NO GROWTH EFFECT ON LACTOBACILLUS CASEI.