Method for cultivation and apparatus therefor

Abstract

Abstract PURPOSE:To efficiently carry out production of useful substances by tissual cultivation, by providing cultivation condition such that a substance to be cultivated may propagate in one of culture vessels and an useful substance may be produced in the other culture vessel and performing specific treatment. CONSTITUTION:This culture device has at least two first and second culture vessels 10 and 20. The respective culture vessels 10 and 20 are designed to continuously or discontinuously communicate the interiors with a main communicating pipe 30. A culture fluid which is part of the culture medium stored in the first culture vessel 10, together with a substance that is to be cultivated and contained in the culture fluid, is suitably transferred from the first culture vessel 10 to the second culture vessel 20. This transfer is carried out using a valve 31 provided in the course of the main communicating pipe 30. The composition components in the culture fluid is mutually varied to especially provide the first culture vessel 10 with culture medium condition suitable for propagation of cells. Thereby the second culture vessel 20 is under condition suitable for production of substances useful for cells.

Inventors

• 👤 Koichi Matsubara
• 👤 Toshihiro Yoshioka
• 👤 Yoshio Motoyama

Bibliographic Details